GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)

Product Name 

GAPDH antibody [mAbcam 9484] - Loading Control

Product type 

Primary antibodies

Description 

Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control

Immunogen 

Full length native protein from human erythrocytes.

Reacts with 
(species key)

Hu, Ms, Rat, Chk, ChHm, Cow, Dog, Pig, Rb, Xl

Specificity 

This antibody shows a single clean band in Western blotting on cell lysates (rat and chinese hamster lysates also show another slightly lower band).

Tested applications 
(see key)

IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-P: Use at a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 40.2 kDa).

NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this antibody following concerning customer feedback on the lack of signal with some of the vials. We found that milk significantly decreases the signal and is therefore not a suitable blocking agent for this antibody (see images of our tests available on the datasheet of the non-HRP conjugated version of the antibody, ab9484). This change in the characteristics of the antibody is due to a recent update to the production process.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Energy Metabolism
Isotype/Loading Controls >> Loading Controls >> GAPDH
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Huntington's disease

Relevance 

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed in almost all tissues at high levels, therefore becoming the marker of choice when a loading control in Western blotting is required. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

mAbcam 9484

Myeloma 

Sp2/0-Ag14

Isotype 

IgG2b

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for GAPDH antibody [mAbcam 9484] - Loading Control images, references, products related to ab9484 and other tools.

GAPDH antibody [mAbcam 9484] - Loading Control images:

References for GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)

  This product has been used in: (two most recent references)

Chevanne M et al. Inhibition of PARP activity by PJ-34 leads to growth impairment and cell death associated with aberrant mitotic pattern and nucleolar actin accumulation in M14 melanoma cell line. J Cell Physiol 222:401-10 (2010). WB; Human. PubMed: 19890834

Zhan DY et al. Therapeutic effect of {beta}-adrenoceptor blockers using a mouse model of dilated cardiomyopathy with a troponin mutation. Cardiovasc Res 84:64-71 (2009). PubMed: 19477965

See all 48 publication references for this product.


If you publish research using ab9484 please let us know so that we can cite the reference on this datasheet.

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