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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
This fast track antibody is not yet fully characterised. It is subject to these terms and conditions
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Proteins and Peptides
ab74478
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Secondary antibodies
Buying a fast track?
We are able to bring you this product at a low price as it is not yet fully characterized. Our Scientific Support team are available to assist you, but we cannot offer refunds or replacements on this product.
What is a fast track? »Publishing research using ab65929? Please let us know so that we can cite the reference in this datasheet
ab65929 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Immunocytochemistry/ Immunofluorescence - GAPex 5 antibody (ab65929)
ICC/IF image of ab65929 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65929, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.This antibody also gave a positive IF result in xxxx cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GAPex 5 antibody (ab65929)
IHC image of ab65929 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65929, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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