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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - GAPex 5 antibody (ab86033)
All lanes : Anti-GAPex 5 antibody (ab86033) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
developed using the ECL technique
Predicted band size : 165 kDa
Observed band size : 180 kDa (why is the actual band size different from the predicted?)
Additional bands at : 71 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
Immunoprecipitation - GAPex 5 antibody (ab86033)
ab86033, at 0.1 µg/ml, staining GAPex 5 in HeLa whole cell lysate immunoprecipitated using ab86033 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: by chemiluminescence with an exposure time of 30 seconds.
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