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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
Overview
Product name
Anti-GAPex 5 antibody
See all GAPex 5 products (2) ...
Description
Rabbit polyclonal to GAPex 5
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
A synthetic peptide corresponding to a region between residue 550 and 600 of human GAPex 5 (BAG10470.1).
Positive control
HeLa whole cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab86033 was affinity purified using an epitope specific to GAPex 5 immobilized on solid support
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Energy Metabolism
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
Applications
Show applications key
Our Abpromise guarantee covers the use of ab86033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IP: Use at 2-5 µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 165 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
GAPex 5 plays a role in several processes such as endocytosis, insulin receptor internalisation and LC2A4/GLUT4 trafficking. It acts as both a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF). GAPex 5 is a GEF for the small G protein Rab31, promoting the exchange of GDP bound to Rab31 for GTP and thus regulating LC2A4/GLUT4 trafficking. In the absence of insulin, GAPex 5 maintains Rab31 in an active (GTP-bound) state, thereby promoting a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, keeping LC2A4/GLUT4 inside the cells. Following insulin stimulation, GAPex 5 is translocated to the plasma membrane, enabling the release of LC2A4/GLUT4 from the intracellular storage vesicles. GAPex 5 is also involved in EGFR trafficking and degradation, and also has GEF activity for Rab5 and GAP activity for Ras.
Cellular localization
Cell Membrane (peripheral membrane protein) and Cytoplasmic
Alternative names
- GAPEX5 antibody
- GAPVD1 antibody
- GTPase activating protein and VPS9 domains 1 antibody
see all
Database links
- Entrez Gene: 26130 Human
- Omim: 611714 Human
- SwissProt: Q14C86 Human
- Unigene: 495134 Human
Anti-GAPex 5 antibody images:
Western blot - GAPex 5 antibody (ab86033)
All lanes : Anti-GAPex 5 antibody (ab86033) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
developed using the ECL technique
Predicted band size : 165 kDa
Observed band size : 180 kDa (why is the actual band size different from the predicted?)
Additional bands at : 71 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
Immunoprecipitation - GAPex 5 antibody (ab86033)
ab86033, at 0.1 µg/ml, staining GAPex 5 in HeLa whole cell lysate immunoprecipitated using ab86033 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: by chemiluminescence with an exposure time of 30 seconds.
References for Anti-GAPex 5 antibody (ab86033)
ab86033 has not yet been referenced specifically in any publications.
Publishing research using ab86033? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"