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Anti-GAS41 antibody [YEATB1A8] (ab50963)

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    1 question for ab50963

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    Question 1

    Monday 05-November-2007

    What was the cell lysated/sample used for ab50963?

    ANSWER:

    		  

    The WB image of ab50963 was obtained by using immunogen peptide with Tag. Unfortunately, we have not obtained the data from cell lysate yet. Please refer the immunogen sequence and protocol as follows for more information.

    1. Immunogen sequence

    HESYGNPLRVVTKPPYEITETGWGEFEIIIKIFFIDPNERPVTLYHLLKLFQSDTNAMLGKKTVVSEFYDEMIFQD PTAMMQQLLTTSRQLTLGAYKHETEFAELEVKTREKLEAA Molecular weight in the calculation is 13.5kD.

    2. MW of Tag: approx. 20kDa

    3. Primary Ab dilution: 1:100

    4. Immunogen amount: 800ng/lane

    5. Secondary Ab dilution: 1:3000

    6. Secondary Ab information: Sheep Anti Mouse IgG, Original Conc.; Antibody: 0.63mg/ml, HRP : 0.78mg/ml

    I hope the above information is helpful for you.

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