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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-GATA1 antibody - ChIP Grade
See all GATA1 products (13) ...
Description
Rabbit polyclonal to GATA1 - ChIP Grade
Tested applications
ChIP, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide: AEAYRHSPVFQVY, corresponding to amino acids 66-78 of Human GATA1.
AEAYRHSPVF QVY
Positive control
K-562 nuclear extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide and 0.01% Thiomerosal.
Constituents: PBS containing 0.2% Gelatin.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Hematopoietic system development
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Western blot - Anti-GATA1 antibody - ChIP Grade (ab11963)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab11963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent concentration.
WB: 1/500 - 1/1000.Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).(Some unspecific bands have been reported with this antibody but it will yield a much stronger signal for GATA-1 at 47 KDa and the antibody may need to be diluted to 1/5000 or more.)
Target
Relevance
GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch from fetal hemoglobin production to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Acquired somatic mutations in GATA1 occur in virtually all children with Down's Syndrome, congenital transient myeloproliferative syndrome (TMD) and acute megakaryocytic leukemia.
Cellular localization
Nuclear
Alternative names
- Anemia, X-linked, without thrombocytopenia, included antibody
- ERYF 1 antibody
- ERYF1 antibody
see all
Database links
- Entrez Gene: 2623 Human
- Entrez Gene: 14460 Mouse
- Entrez Gene: 25172 Rat
- Omim: 305371 Human
- SwissProt: P15976 Human
- SwissProt: P17679 Mouse
- SwissProt: Q3UIH9 Mouse
- SwissProt: Q7TMX8 Mouse
see all
Anti-GATA1 antibody - ChIP Grade images:
Western blot - Anti-GATA1 antibody - ChIP Grade (ab11963)
Predicted band size : 43 kDa
Detection of GATA1 by Western blot analysis. Antibody titration experiments using GATA1 rabbit polyclonal antibody in nuclear extracts derived from K562 cells (lanes 1 to 3 - dilutions 1/500, 1/1000 and 1/2000 respectively). The signal corresponding to nuclear GATA1 is eliminated when the immunizing peptide (lane 4) is added to the reaction mixture.
Detection of GATA1 by Western blot analysis. Antibody titration experiments using GATA1 rabbit polyclonal antibody in nuclear extracts derived from K562 cells (lanes 1 to 3 - dilutions 1/500, 1/1000 and 1/2000 respectively). The signal corresponding to nuclear GATA1 is eliminated when the immunizing peptide (lane 4) is added to the reaction mixture.
References for Anti-GATA1 antibody - ChIP Grade (ab11963)
This product has been referenced in:
- Kassouf MTet al. Genome-wide identification of TAL1's functional targets: insights into its mechanisms of action in primary erythroid cells. Genome Res 20:1064-83 (2010). CHIPseq; Mouse.Read more (PubMed: 20566737) »
- Ogilvy Set al. The SCL +40 enhancer targets the midbrain together with primitive and definitive hematopoiesis and is regulated by SCL and GATA proteins. Mol Cell Biol 27:7206-19 (2007). ChIP; Mouse.Read more (PubMed: 17709394) »
See all 2 publications for this product
Publishing research using ab11963? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"