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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-GATA1 antibody
See all GATA1 products (13) ...
Description
Rabbit polyclonal to GATA1
Tested applications
CHIPseq, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: K-FPTGPMPPTTSTTVVAPLSS, with N-terminal added Lysine, conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 394-413 of Human GATA1.
K-FPTGPMPP TTSTTVVAPL SS
Positive control
K562 cell line.
General notes
Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilution samples should be discarded if not used within 12 hours.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
CHIPseq: Use at an assay dependent concentration. (PubMed: 21795385)
WB: 1/400Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).(This concentration is determined using whole extract of K562 human chronic myelogenous leukemia cells. Detects a band of approximately 45 kDa. An additional lower molecular weight band may appear in some preparations. Staining of the GATA1 band is inhibited by the GATA1 peptide (amino acid residues 394-413))
Target
Relevance
GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch from fetal hemoglobin production to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Acquired somatic mutations in GATA1 occur in virtually all children with Down's Syndrome, congenital transient myeloproliferative syndrome (TMD) and acute megakaryocytic leukemia.
Cellular localization
Nuclear
Alternative names
- Anemia, X-linked, without thrombocytopenia, included antibody
- ERYF 1 antibody
- ERYF1 antibody
see all
Database links
- Entrez Gene: 2623 Human
- Omim: 305371 Human
- SwissProt: P15976 Human
- Unigene: 765 Human
References for Anti-GATA1 antibody (ab11852)
This product has been referenced in:
- Hu Get al. Regulation of nucleosome landscape and transcription factor targeting at tissue-specific enhancers by BRG1. Genome Res 21:1650-8 (2011). CHIPseq; Human.Read more (PubMed: 21795385) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"