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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-GATA1 antibody
See all GATA1 products (13) ...
Description
Rabbit polyclonal to GATA1
Specificity
ab28839 recognises GATA-1.
Tested applications
WB, ELISA, IHC-P, IP, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic non-phosphopeptide derived from human GATA1 around the phosphorylation site of Serine 142.
Positive control
IHC: breast carcinoma WB: K562 cell line
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg++ and Ca++), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28839 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
ELISA
ELISA: 1/10000ELISA: 1/10000
IHC-P: 1/50 - 1/100.
IP: Use at an assay dependent dilution.
ICC/IF: Use a concentration of 1 - 5 µg/ml.
Target
Relevance
GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch from fetal hemoglobin production to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Acquired somatic mutations in GATA1 occur in virtually all children with Down's Syndrome, congenital transient myeloproliferative syndrome (TMD) and acute megakaryocytic leukemia.
Cellular localization
Nuclear
Alternative names
- Anemia, X-linked, without thrombocytopenia, included antibody
- ERYF 1 antibody
- ERYF1 antibody
see all
Database links
- Entrez Gene: 2623 Human
- Entrez Gene: 14460 Mouse
- Omim: 305371 Human
- SwissProt: P15976 Human
- SwissProt: P17679 Mouse
- SwissProt: Q3UIH9 Mouse
- SwissProt: Q7TMX8 Mouse
- Unigene: 765 Human
Anti-GATA1 antibody images:
Immunohistochemistry (Paraffin-embedded sections) - GATA1 antibody (ab28839)
Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using GATA-1(Ab-1) Antibody (ab28839); Right: The same antibody preincubated with synthesized peptide.
Western blot - GATA1 antibody (ab28839)
All lanes : Anti-GATA1 antibody (ab28839) (1/500-1/1000 dilution)
Lane 1 : extracts from K562 cells (5-30ug).
Lane 2 : extracts from K562 cells (5-30ug).
Predicted band size : 43 kDa
Observed band size : 43 kDa
Western blot analysis of extracts from K562 cells. Line1: Using GATA-1 antibody (ab28839); Line2: The same antibody preincubated with synthesized peptide.
Immunocytochemistry/ Immunofluorescence - GATA1 antibody (ab28839)
ICC/IF image of ab28839 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28839, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-GATA1 antibody (ab28839)
This product has been referenced in:
- Boidot Ret al. The transcription factor GATA-1 is overexpressed in breast carcinomas and contributes to survivin upregulation via a promoter polymorphism. Oncogene : (2010). WB, IHC-P; Human.Read more (PubMed: 20101202) »
- Hoeller Set al. New GATA1 mutation in codon 2 leads to the earliest known premature stop codon in transient myeloproliferative disorder. Blood 114:3717-8 (2009). IHC-P; Human.Read more (PubMed: 19850750) »
See all 3 publications for this product
Publishing research using ab28839? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"