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Immunohistochemistry (Paraffin-embedded sections) - GATA1 antibody (ab28839)
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Western blot - GATA1 antibody (ab28839)
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Immunocytochemistry/ Immunofluorescence-GATA1 antibody(ab28839)
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Product Name
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GATA1 antibody
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See all GATA1 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to GATA1
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Immunogen
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Synthetic non-phosphopeptide derived from human GATA1 around the phosphorylation site of Serine 142.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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ab28839 recognises GATA-1.
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
ICC/IF: Use at a concentration of 1 - 5 µg/ml.
IHC-P: 1/50 - 1/100.
IP: Use at an assay dependent dilution.
WB: 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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IHC: breast carcinoma
WB: K562 cell line
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Organogenesis >> Hematopoietic system development
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Relevance
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GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch from fetal hemoglobin production to adult hemoglobin.
Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Acquired somatic mutations in GATA1 occur in virtually all children with Down's Syndrome, congenital transient myeloproliferative syndrome (TMD) and acute megakaryocytic leukemia.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg++ and Ca++), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GATA1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GATA1 antibody images, references, products related to ab28839 and other tools.
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GATA1 antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - GATA1 antibody (ab28839)
Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using GATA-1(Ab-1) Antibody (ab28839); Right: The same antibody preincubated with
synthesized peptide.
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Western blot - GATA1 antibody (ab28839)
All lanes : GATA1 antibody (ab28839) (1/500-1/1000 dilution)
Lane 1 : extracts from K562 cells (5-30ug).
Lane 2 : extracts from K562 cells (5-30ug).
Predicted band size : 43 kDa
Observed band size : 43 kDa
Western blot analysis of extracts from K562 cells. Line1: Using GATA-1 antibody (ab28839); Line2: The same antibody preincubated with synthesized peptide.
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Immunocytochemistry/ Immunofluorescence-GATA1 antibody(ab28839)
ICC/IF image of ab28839 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28839, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for GATA1 antibody (ab28839)
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This product has been used in: (two most recent references)
Boidot R et al. The transcription factor GATA-1 is overexpressed in breast carcinomas and contributes to survivin upregulation via a promoter polymorphism. Oncogene : (2010). WB, IHC-P; Human. PubMed: 20101202
Hoeller S et al. New GATA1 mutation in codon 2 leads to the earliest known premature stop codon in transient myeloproliferative disorder. Blood 114:3717-8 (2009). IHC-P; Human. PubMed: 19850750
See all 3 publication references for this product.
If you publish research using ab28839 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to GATA1
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GATA1 antibody - more information
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