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Western blot - GATA1 antibody (ab40847)
(enlarge)
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Product Name
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GATA1 antibody
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See all GATA1 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to GATA1
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Immunogen
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Synthetic peptide: DAEAYRHSPVFQ, corresponding to Internal sequence amino acids 65-76 of Human GATA1
BLAST 'DAEAYRHSPVFQ' with  or 
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Reacts with
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Hu
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Predicted to react
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Ms, Rat, Dog
(Due to sequence homology)
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Tested applications
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ELISA, WB
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Abreviews
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Application notes
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Recommended dilutions
Peptide ELISA: Antibody detection limit dilution 1:16,000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 43 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Organogenesis >> Hematopoietic system development
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Relevance
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GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch from fetal hemoglobin production to adult hemoglobin.
Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Acquired somatic mutations in GATA1 occur in virtually all children with Down's Syndrome, congenital transient myeloproliferative syndrome (TMD) and acute megakaryocytic leukemia.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GATA1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GATA1 antibody images, references, products related to ab40847 and other tools.
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GATA1 antibody images:
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 Western blot - GATA1 antibody (ab40847)
GATA1 antibody (ab40847) at 0.3 µg/ml + Human PBMC lysate (35µg protein in RIPA buffer).
Predicted band size : 43 kDa
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
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Search PubMed (MEDLINE) for references to GATA1
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GATA1 antibody - more information
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Customer reviews (feedback) regarding GATA1 antibody |
Customer FAQs regarding GATA1 antibody |
Protocols for GATA1 antibody |
Price and availability of products related to GATA1 antibody |
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GATA1 antibody - related products:
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GATA1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about GATA1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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