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ab28816 at (1:50-1:100), staining paraffin embedded human breast carcinoma. Left: Using GATA1 antibody (ab28816); Right: antibody preincubated with synthesized phosphopeptide.
Lane 1 : Anti-GATA1 (phospho S142) antibody (ab28816) at 1/500 dilution
Lane 2 : Anti-GATA1 (phospho S142) antibody (ab28816) at 1/500 dilution (preincubated with synthesized non-phosphopeptide)
Lane 3 : Anti-GATA1 (phospho S142) antibody (ab28816) at 1/500 dilution (preincubated with synthesized phosphopeptide)
Lane 1 : K562 cells
Lane 2 : K562 cells
Lane 3 : K562 cells
Observed band size : 43 kDa (why is the actual band size different from the predicted?)
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