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Anti-GATA1 (phospho S142) antibody (ab28816)

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1 question for ab28816

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Question 1

Thursday 06-July-2006

Customer is seeing 3 bands on Western blot on bone marrow. ~50, 46, 40 kDa. The protein should be about 43 kDa.

ANSWER:

  

Thank you for your enquiry.

For this antibody, we saw a single band on Western blot when using k562 cell lysates. We did not test this antibody on bone marrow.

1) Did you use Tween-20 in the buffers? Tween-20 (0.05%) was added to the primary and secondary antibody solutions and wash buffer in our protocol to reduce non-specific bands. If so, increasing the stringency for Western blot (using a higher concentration of Tween-20 and washing for longer) can reduce non-specific bands.

2) To identify which is the correct band on your Western blot with bone marrow samples, I would recommend using k562 cell lysates as a positive control.

I hope this information helps. Please do not hesitate to contact us if you need anything further.

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