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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-GATA1 (phospho S142) antibody
See all GATA1 products (13) ...
Description
Rabbit polyclonal to GATA1 (phospho S142)
Tested applications
IHC-P, WB, ELISA, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic phosphopeptide derived from human GATA1 around the phosphorylation site of Serine 142.
Positive control
k562 cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
After immunogen affinity purification the antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000.Detects a band of approximately 43 kDa.
ELISA
ELISA: 1/4000ELISA: 1/4000
IP: Use at an assay dependent dilution.
Target
Relevance
GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch from fetal hemoglobin production to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Acquired somatic mutations in GATA1 occur in virtually all children with Down's Syndrome, congenital transient myeloproliferative syndrome (TMD) and acute megakaryocytic leukemia.
Cellular localization
Nuclear
Alternative names
- Anemia, X-linked, without thrombocytopenia, included antibody
- ERYF 1 antibody
- ERYF1 antibody
see all
Database links
- Entrez Gene: 2623 Human
- Entrez Gene: 14460 Mouse
- Omim: 305371 Human
- SwissProt: P15976 Human
- SwissProt: P17679 Mouse
- SwissProt: Q3UIH9 Mouse
- SwissProt: Q7TMX8 Mouse
- Unigene: 765 Human
Anti-GATA1 (phospho S142) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - GATA1 (phospho S142) antibody (ab28816)
ab28816 at (1:50-1:100), staining paraffin embedded human breast carcinoma. Left: Using GATA1 antibody (ab28816); Right: antibody preincubated with synthesized phosphopeptide.
Western blot - GATA1 antibody (ab28816)
Lane 1 : Anti-GATA1 (phospho S142) antibody (ab28816) at 1/500 dilution
Lane 2 : Anti-GATA1 (phospho S142) antibody (ab28816) at 1/500 dilution (preincubated with synthesized non-phosphopeptide)
Lane 3 : Anti-GATA1 (phospho S142) antibody (ab28816) at 1/500 dilution (preincubated with synthesized phosphopeptide)
Lane 1 : K562 cells
Lane 2 : K562 cells
Lane 3 : K562 cells
Observed band size : 43 kDa (why is the actual band size different from the predicted?)
References for Anti-GATA1 (phospho S142) antibody (ab28816)
ab28816 has not yet been referenced specifically in any publications.
Publishing research using ab28816? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"