GATA1 (phospho S142) antibody (ab28816)

Product Name 

GATA1 (phospho S142) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to GATA1 (phospho S142)

Immunogen 

Synthetic phosphopeptide derived from human GATA1 around the phosphorylation site of Serine 142.

Reacts with 
(species key)

Hu, Ms

Tested applications 
(see key)

ELISA, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/4000.
IHC-P: 1/50 - 1/100.
IP: Use at an assay dependent dilution.
WB: 1/500 - 1/1000. Detects a band of approximately 43 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

k562 cell lysate

Cellular localization 

Nuclear

Research areas 

Developmental Biology >> Organogenesis >> Hematopoietic system development
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger

Relevance 

GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch from fetal hemoglobin production to adult hemoglobin.
Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Acquired somatic mutations in GATA1 occur in virtually all children with Down's Syndrome, congenital transient myeloproliferative syndrome (TMD) and acute megakaryocytic leukemia.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

After immunogen affinity purification the antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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