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All lanes : Anti-GATA1 (phospho S310) antibody (ab36719) at 1/500 dilution
Lane 1 : extracts from COS-7 cells
Lane 2 : extracts from COS-7 cells treated with EPO
Lysates/proteins at 30 µg per lane.
Predicted band size : 43 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Lanes can be loaded with 5-30µg of total protein.
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