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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-GATA1 (phospho S310) antibody
See all GATA1 products (13) ...
Description
Rabbit polyclonal to GATA1 (phospho S310)
Specificity
This antibody detects endogenous levels of GATA1 only when phosphorylated at Serine 310.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human GATA1 around the phosphorylation site of Serine 310 (K-A-SP-G-K).
Positive control
COS7 cell extracts.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg++ and Ca++), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - GATA1 (phospho S310) antibody (ab36719)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab36719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 43 kDa.
ELISA
ELISA: 1/20000ELISA: 1/20000
Target
Relevance
GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch from fetal hemoglobin production to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Acquired somatic mutations in GATA1 occur in virtually all children with Down's Syndrome, congenital transient myeloproliferative syndrome (TMD) and acute megakaryocytic leukemia.
Cellular localization
Nuclear
Alternative names
- Anemia, X-linked, without thrombocytopenia, included antibody
- ERYF 1 antibody
- ERYF1 antibody
see all
Database links
- Entrez Gene: 2623 Human
- Entrez Gene: 14460 Mouse
- Entrez Gene: 25172 Rat
- Omim: 305371 Human
- SwissProt: P15976 Human
- SwissProt: P17679 Mouse
- SwissProt: Q3UIH9 Mouse
- SwissProt: Q7TMX8 Mouse
see all
Anti-GATA1 (phospho S310) antibody images:
Western blot - GATA1 (phospho S310) antibody (ab36719)
All lanes : Anti-GATA1 (phospho S310) antibody (ab36719) at 1/500 dilution
Lane 1 : extracts from COS-7 cells
Lane 2 : extracts from COS-7 cells treated with EPO
Lysates/proteins at 30 µg per lane.
Predicted band size : 43 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Lanes can be loaded with 5-30µg of total protein.
References for Anti-GATA1 (phospho S310) antibody (ab36719)
ab36719 has not yet been referenced specifically in any publications.
Publishing research using ab36719? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"