Anti-GATA1 (phospho S310) antibody (ab36719)

Overview

  • Product nameAnti-GATA1 (phospho S310) antibody
    See all GATA1 primary antibodies
  • Description
    Rabbit polyclonal to GATA1 (phospho S310)
  • SpecificityThis antibody detects endogenous levels of GATA1 only when phosphorylated at Serine 310.
  • Tested applicationsICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    The antiserum was produced against synthesized phosphopeptide derived from human GATA1 around the phosphorylation site of Serine 310 (K-A-SP-G-K).

  • Positive control
    • COS7 cell extracts. IF/ICC: MCF7 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab36719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 43 kDa.
ELISA 1/20000.

Target

  • FunctionTranscriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells.
  • Tissue specificityErythrocytes.
  • Involvement in diseaseDefects in GATA1 are the cause of X-linked dyserythropoietic anemia and thrombocytopenia (XDAT) [MIM:300367]. XDAT is a disorder characterized by erythrocytes with abnormal size and shape, and paucity of platelets in peripheral blood. The bone marrow contains abundant and abnormally small megakaryocytes.
    Defects in GATA1 are the cause of X-linked thrombocytopenia with beta-thalassemia (XLTT) [MIM:314050]; also knwon as thrombocytopenia, platelet dysfunction, hemolysis, and imbalanced globin synthesis. XLTT consists of an unusual form of thrombocytopenia with beta-thalassemia. Patients have splenomegaly and petechiae, moderate thrombocytopenia, prolonged bleeding time due to platelet dysfunction, reticulocytosis and unbalanced hemoglobin chain synthesis resembling that of beta-thalassemia minor.
    Defects in GATA1 are the cause of anemia without thrombocytopenia X-linked (XLAWT) [MIM:300835]. XLAWT is a form of anemia characterized by abnormal morphology of erythrocytes and granulocytes in peripheral blood, bone marrow dysplasia with hypocellularity of erythroid and granulocytic lineages, and normal or increased number of megakaryocytes. Neutropenia of a variable degree is present in affected individuals.
  • Sequence similaritiesContains 2 GATA-type zinc fingers.
  • DomainThe two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding.
  • Post-translational
    modifications
    Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137.
    Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation by SUMO1 has no effect on transcriptional activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anemia, X-linked, without thrombocytopenia, included antibody
    • ERYF 1 antibody
    • ERYF1 antibody
    • ERYF1 antibody
    • Erythroid transcription factor antibody
    • Erythrold transcription factor 1 antibody
    • Erythrold transcription factor 1 antibody
    • GATA 1 antibody
    • GATA binding factor 1 antibody
    • GATA binding protein 1 (globin transcription factor 1) antibody
    • GATA binding protein 1 antibody
    • GATA binding protein 1 antibody
    • GATA-1 antibody
    • GATA-binding factor 1 antibody
    • GATA1 antibody
    • GATA1_HUMAN antibody
    • GF 1 antibody
    • GF-1 antibody
    • GF1 antibody
    • GF1 antibody
    • Globin transcription factor 1 antibody
    • Globin transcription factor 1 antibody
    • NF E1 antibody
    • NF E1 DNA binding protein antibody
    • NF-E1 DNA-binding protein antibody
    • NFE 1 antibody
    • NFE1 antibody
    • NFE1 antibody
    • Nuclear factor erythroid 1 antibody
    • Transcription factor GATA1 antibody
    • XLANP antibody
    • XLTDA antibody
    • XLTT antibody
    see all

Anti-GATA1 (phospho S310) antibody images

  • ICC/IF image of ab36719 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36719, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-GATA1 (phospho S310) antibody (ab36719) at 1/500 dilution

    Lane 1 : extracts from COS-7 cells
    Lane 2 : extracts from COS-7 cells treated with EPO

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 43 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Lanes can be loaded with 5-30µg of total protein.

References for Anti-GATA1 (phospho S310) antibody (ab36719)

ab36719 has not yet been referenced specifically in any publications.

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