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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-GATA2 antibody - ChIP Grade
See all GATA2 products (4) ...
Description
Rabbit polyclonal to GATA2 - ChIP Grade
Tested applications
EMSA, ChIP, IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Dog, Pig
Immunogen
Synthetic peptide: PYYANPAHAR ARVSYSPAHA RLTGGQMCRP HLLHSPGLPW LDGGKAALSA , corresponding to N terminal amino acids 58-107 of Human GATA2
PYYANPAHAR ARVSYSPAHA RLTGGQMCRP HLLHSPGLPW LDGGKAALSA
Positive control
Human liver tissue (IHC-P), K682 cell lysate (western blot).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Lineage specification >> Ectoderm
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Stem Cells >> Lineage Markers >> Ectoderm
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22849 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EMSA: Use at an assay dependent dilution. (PubMed: 20599521)
ChIP: Use at an assay dependent dilution.
IHC-P: Use a concentration of 4 - 8 µg/ml.
WB: Use a concentration of 1 - 2 µg/ml.Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
Target
Function
Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'.
Tissue specificity
Endothelial cells.
Sequence similarities
Contains 2 GATA-type zinc fingers.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP23769
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Endothelial transcription factor GATA 2 antibody
- Endothelial transcription factor GATA-2 antibody
- Endothelial transcription factor GATA2 antibody
see all
Database links
- Entrez Gene: 2624 Human
- Entrez Gene: 14461 Mouse
- Entrez Gene: 396912 Pig
- Entrez Gene: 25159 Rat
- Omim: 137295 Human
- SwissProt: P23824 Chicken
- SwissProt: P23769 Human
- SwissProt: Q96BH0 Human
see all
Anti-GATA2 antibody - ChIP Grade images:
Western blot - Gata2 antibody (ab22849)
Anti-GATA2 antibody - ChIP Grade (ab22849) at 4 µg/ml + K682 (liver) cell lysate
Predicted band size : 50 kDa
Observed band size : 50 kDa
Gel concentration: 12%
Immunohistochemistry (Paraffin-embedded sections) - Gata2 antibody (ab22849)
Paraffin-embedded human liver tissue. Cells with positive label: hepatocytes (indicated with arrows). Magnification: 400x
References for Anti-GATA2 antibody - ChIP Grade (ab22849)
This product has been referenced in:
- Jack BH & Crossley M GATA proteins work together with friend of GATA (FOG) and C-terminal binding protein (CTBP) co-regulators to control adipogenesis. J Biol Chem 285:32405-14 (2010). WB; Human.Read more (PubMed: 20705609) »
- Huang YJet al. The functional IGFBP7 promoter -418G>A polymorphism and risk of head and neck cancer. Mutat Res 702:32-9 (2010). EMSA; Human.Read more (PubMed: 20599521) »
See all 3 publications for this product
Publishing research using ab22849? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"