GATA2 antibody - ChIP Grade (ab22849)

Product Name 

GATA2 antibody - ChIP Grade

Product type 

Primary antibodies

Description 

Rabbit polyclonal to GATA2 - ChIP Grade

Immunogen 

A region within synthetic peptide: AQLLPPDEVD VFFNHLDSQG NPYYANPAHA RARVSYSPAH ARLTGGQMCR, corresponding to N terminal amino acids 37-86 of Human GATA2
BLAST 'AQLLPPDEVD VFFNHLDSQG NPYYANPAHA RARVSYSPAH ARLTGGQMCR ' with or

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ChIP, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ChIP: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human liver tissue (IHC-P), K682 cell lysate (western blot).

Cellular localization 

Nuclear

Research areas 

Developmental Biology >> Lineage specification >> Ectoderm
Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Stem Cells >> Lineage Markers >> Ectoderm
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger

Relevance 

GATA2 is expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells. In chicken erythroid progenitors, forced expression of GATA2 promotes proliferation at the expense of differentiation (Briegel et al., 1993). GATA2 plays a critical role in hematopoiesis. Primitive hematopoiesis in the yolk sac of mutant mice was greatly reduced and led to severe anaemia and death. Definitive hematopoiesis, evident in fetal liver followed by maintenance in the spleen and then adult bone marrow, was also significantly affected (greater than 50-fold). These data are most consistent with GATA2 serving as a regulator of genes controlling growth factor responsiveness or proliferative capacity of early hematopoietic cells.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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GATA2 antibody - ChIP Grade images:

References for GATA2 antibody - ChIP Grade (ab22849)

  This product has been used in:

Mammoto A et al. A mechanosensitive transcriptional mechanism that controls angiogenesis. Nature 457:1103-8 (2009). ChIP; Human. PubMed: 19242469

If you publish research using ab22849 please let us know so that we can cite the reference on this datasheet.

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