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ab85817 |
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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Anti-GATA2 antibody
See all GATA2 products (4) ...
Rabbit polyclonal to GATA2
Reacts with
Mouse, Rat
Predicted to work with
Horse, Cow, Dog, Human, Pig, Zebrafish, Rhesus monkey, Zebra finch
Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human GATA2.
(Peptide available as ab85817.)
This antibody gave a positive signal in both Rat and Mouse Liver Tissue Lysates
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Western blot - GATA2 antibody (ab70366)
(enlarge)
Our Abpromise guarantee covers the use of ab70366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'.
Endothelial cells.
Contains 2 GATA-type zinc fingers.
Nucleus.
Target information above from: UniProt accessionP23769
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - GATA2 antibody (ab70366)

All lanes : Anti-GATA2 antibody (ab70366) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
ab70366 has not yet been referenced specifically in any publications.
Publishing research using ab70366? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:

All lanes : Anti-GATA2 antibody (ab70366) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
0
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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