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Western blot - GATA2 antibody (ab70366)

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Product Name
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GATA2 antibody
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See all GATA2 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to GATA2
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human GATA2.
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Reacts with
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Ms, Rat
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Predicted to react
(species key)
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Hu, Cow, Dog, Hrs, Pig, RMk, Zfnh (Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in both Rat and Mouse Liver Tissue Lysates
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Lineage specification >> Ectoderm Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators Stem Cells >> Lineage Markers >> Ectoderm Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Relevance
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GATA2 is expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells. In chicken erythroid progenitors, forced expression of GATA2 promotes proliferation at the expense of differentiation (Briegel et al., 1993). GATA2 plays a critical role in hematopoiesis. Primitive hematopoiesis in the yolk sac of mutant mice was greatly reduced and led to severe anaemia and death. Definitive hematopoiesis, evident in fetal liver followed by maintenance in the spleen and then adult bone marrow, was also significantly affected (greater than 50-fold). These data are most consistent with GATA2 serving as a regulator of genes controlling growth factor responsiveness or proliferative capacity of early hematopoietic cells.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.800 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GATA2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GATA2 antibody images, references, products related to ab70366 and other tools.
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GATA2 antibody images:
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Western blot - GATA2 antibody (ab70366)
All lanes : GATA2 antibody (ab70366) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate Lane 2 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 50 kDa Observed band size : 55 kDa (why is the actual band size different from the predicted?) Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to GATA2
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GATA2 antibody - more information
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Customer reviews (feedback) regarding GATA2 antibody |
Customer FAQs regarding GATA2 antibody |
Protocols for GATA2 antibody |
Price and availability of products related to GATA2 antibody |
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GATA2 antibody - related products:
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GATA2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about GATA2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"