GATA2 antibody (ab70366)

Product Name 

GATA2 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to GATA2

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human GATA2.

Reacts with 

Ms, Rat

Predicted to react 
(species key)

Hu, Cow, Dog, Hrs, Pig, RMk, Zfnh
(Due to sequence homology)

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

This antibody gave a positive signal in both Rat and Mouse Liver Tissue Lysates

Cellular localization 

Nuclear

Research areas 

Developmental Biology >> Lineage specification >> Ectoderm
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Stem Cells >> Lineage Markers >> Ectoderm
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger

Relevance 

GATA2 is expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells. In chicken erythroid progenitors, forced expression of GATA2 promotes proliferation at the expense of differentiation (Briegel et al., 1993). GATA2 plays a critical role in hematopoiesis. Primitive hematopoiesis in the yolk sac of mutant mice was greatly reduced and led to severe anaemia and death. Definitive hematopoiesis, evident in fetal liver followed by maintenance in the spleen and then adult bone marrow, was also significantly affected (greater than 50-fold). These data are most consistent with GATA2 serving as a regulator of genes controlling growth factor responsiveness or proliferative capacity of early hematopoietic cells.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.800 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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