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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-GATA2 antibody
See all GATA2 products (4) ...
Description
Rabbit polyclonal to GATA2
Tested applications
Cross reactivity
Reacts with
Mouse, Rat
Predicted to work with
Horse, Cow, Dog, Human, Pig, Zebrafish, Rhesus monkey, Zebra finch
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human GATA2.
(Peptide available as ab85817.)
Positive control
This antibody gave a positive signal in both Rat and Mouse Liver Tissue Lysates
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - GATA2 antibody (ab70366)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
Target
Function
Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'.
Tissue specificity
Endothelial cells.
Sequence similarities
Contains 2 GATA-type zinc fingers.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP23769
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Endothelial transcription factor GATA 2 antibody
- Endothelial transcription factor GATA-2 antibody
- Endothelial transcription factor GATA2 antibody
see all
Database links
- Entrez Gene: 2624 Human
- Entrez Gene: 14461 Mouse
- Entrez Gene: 396912 Pig
- Entrez Gene: 25159 Rat
- Entrez Gene: 30480 Zebrafish
- Omim: 137295 Human
- SwissProt: P23769 Human
- SwissProt: Q96BH0 Human
see all
Anti-GATA2 antibody images:
Western blot - GATA2 antibody (ab70366)
All lanes : Anti-GATA2 antibody (ab70366) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
References for Anti-GATA2 antibody (ab70366)
ab70366 has not yet been referenced specifically in any publications.
Publishing research using ab70366? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"