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ab32857
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab32858? Please let us know so that we can cite the reference in this datasheet
ab32858 has been referenced in 1 publications.
- Asselin-Labat ML et al. Gata-3 negatively regulates the tumor-initiating capacity of mammary luminal progenitor cells and targets the putative tumor suppressor caspase-14. Mol Cell Biol 31:4609-22 (2011). PubMed: 21930782
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - GATA3 antibody (ab32858)
All lanes : Anti-GATA3 antibody (ab32858) at 1 µg/ml
Lane 1 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 48 kDa
Observed band size : 51 kDa (why is the actual band size different from the predicted?)
Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GATA3 antibody (ab32858)
ab32858 staining GATA3 in human thymus tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C (5 minutes for peroxidase block and 10 minutes for protein block). The primary antibody was diluted 1/50 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
This antibody is part of an abreview submitted by Antibody Solutions Ltd.
Western blot - GATA3 antibody (ab32858)
All lanes : Anti-GATA3 antibody (ab32858) at 1 µg/ml
Lane 1 : Thymus (Mouse) Tissue Lysate
Lane 2 : Spleen (Mouse) Tissue Lysate
Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 48 kDa
Observed band size : 51 kDa (why is the actual band size different from the predicted?)
Additional bands at : 42 kDa,90 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
GATA3 antibody for WB in Mouse (32858)
GATA3 antibody for IHC-P in Human (32858)
GATA3 antibody for Western blot in Mouse (32858)
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