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Products:Immunology >> Adaptive Immunity >> T Cells >> Non-CD
Overview
Product name
Anti-GATA3 antibody
See all GATA3 products (14) ...
Description
Rabbit polyclonal to GATA3
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human GATA3.
(Peptide available as ab32857.)
Positive control
ab32858 gave a positive signal in the following lysates: MOLT4 (Human acute lymphoblastic leukemia cell line); Jurkat (Human T cell lymphoblast-like cell line); Ramos (Human Burkitt's lymphoma cell line); MEF1(Mouse embryonic fibroblast cell line); Spleen (Mouse) Tissue Lysate; Thymus (Mouse) Tissue Lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Lineage specification >> Ectoderm
Stem Cells >> Lineage Markers >> Ectoderm
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Immunology >> Adaptive Immunity >> T Cells >> Non-CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IHC-P: Use at a dilution of 1/50-1/100 for 1 hr.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
Tissue specificity
T-cells and endothelial cells.
Involvement in disease
Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
Sequence similarities
Contains 2 GATA-type zinc fingers.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP23771
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Form
There are 2 isoforms produced by alternative splicing.
Alternative names
- GATA 3 antibody
- GATA binding factor 3 antibody
- GATA binding protein 3 antibody
see all
Database links
- Entrez Gene: 2625 Human
- Entrez Gene: 14462 Mouse
- Omim: 131320 Human
- SwissProt: P23771 Human
- SwissProt: P23772 Mouse
- Unigene: 524134 Human
- Unigene: 313866 Mouse
Anti-GATA3 antibody images:
Western blot - GATA3 antibody (ab32858)
All lanes : Anti-GATA3 antibody (ab32858) at 1 µg/ml
Lane 1 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 48 kDa
Observed band size : 51 kDa (why is the actual band size different from the predicted?)
Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GATA3 antibody (ab32858)
ab32858 staining GATA3 in human thymus tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C (5 minutes for peroxidase block and 10 minutes for protein block). The primary antibody was diluted 1/50 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
This antibody is part of an abreview submitted by Antibody Solutions Ltd.
Western blot - GATA3 antibody (ab32858)
All lanes : Anti-GATA3 antibody (ab32858) at 1 µg/ml
Lane 1 : Thymus (Mouse) Tissue Lysate
Lane 2 : Spleen (Mouse) Tissue Lysate
Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 48 kDa
Observed band size : 51 kDa (why is the actual band size different from the predicted?)
Additional bands at : 42 kDa,90 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
References for Anti-GATA3 antibody (ab32858)
This product has been referenced in:
- Asselin-Labat MLet al. Gata-3 negatively regulates the tumor-initiating capacity of mammary luminal progenitor cells and targets the putative tumor suppressor caspase-14. Mol Cell Biol 31:4609-22 (2011).Read more (PubMed: 21930782) »
See 1 publication for this product
Publishing research using ab32858? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"