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Western blot - GATA3 antibody (ab32858)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GATA3 antibody (ab32858)
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Product Name
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GATA3 antibody
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See all GATA3 antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to GATA3
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human GATA3. (Peptide available as ab32857.)
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms
(Due to sequence homology)
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a dilution of 1/50-1/100 for 1 hr.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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ab32858 gave a positive signal in the following whole cell lysates:
MOLT4 (Human acute lymphoblastic leukemia cell line)
Jurkat (Human T cell lymphoblast-like cell line)
Ramos (Human Burkitt's lymphoma cell line)
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Lineage specification >> Ectoderm
Stem Cells >> Lineage Markers >> Ectoderm
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Immunology >> Adaptive Immunity >> T Cells >> Non-CD
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Relevance
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The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.20 - 0.50 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GATA3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GATA3 antibody images, references, products related to ab32858 and other tools.
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GATA3 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GATA3 antibody (ab32858)
ab32858 staining GATA3 in human thymus tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C (5 minutes for peroxidase block and 10 minutes for protein block). The primary antibody was diluted 1/50 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as secondary. This antibody is part of an abreview submitted by Antibody Solutions Ltd.
See Abreview
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Search PubMed (MEDLINE) for references to GATA3
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GATA3 antibody - more information
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Customer reviews (feedback) regarding GATA3 antibody |
Customer FAQs regarding GATA3 antibody |
Protocols for GATA3 antibody |
Price and availability of products related to GATA3 antibody |
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GATA3 antibody - related products:
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GATA3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about GATA3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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