Anti-GATM antibody [OTI1C9] (ab119269)
Key features and details
- Mouse monoclonal [OTI1C9] to GATM
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-GATM antibody [OTI1C9]
See all GATM primary antibodies -
Description
Mouse monoclonal [OTI1C9] to GATM -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human GATM. Produced in HEK293 cells (NP_001473).
Database link: P50440 -
Positive control
- WB: HEK-293T transfected lysate. IHC-P: Human kidney, liver, liver carcinoma or pancreas tissue Flow Cyt (Intra): HEK-293T cells. ICC/IF: COS-7 transfected cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, PBS -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI1C9 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 48 kDa.
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IHC-P |
1/150.
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ICC/IF |
1/100.
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Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 48 kDa. |
IHC-P
1/150. |
ICC/IF
1/100. |
Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Catalyzes the biosynthesis of guanidinoacetate, the immediate precursor of creatine. Creatine plays a vital role in energy metabolism in muscle tissues. May play a role in embryonic and central nervous system development. May be involved in the response to heart failure by elevating local creatine synthesis. -
Tissue specificity
Expressed in brain, heart, kidney, liver, lung, salivary gland and skeletal muscle tissue, with the highest expression in kidney. Biallelically expressed in placenta and fetal tissues. -
Pathway
Amine and polyamine biosynthesis; creatine biosynthesis; creatine from L-arginine and glycine: step 1/2. -
Involvement in disease
Defects in GATM are the cause of arginine:glycine amidinotransferase deficiency (AGAT deficiency) [MIM:612718]. AGAT deficiency is an autosomal recessive disorder characterized by developmental delay/regression, mental retardation, severe disturbance of expressive and cognitive speech, and severe depletion of creatine/phosphocreatine in the brain. -
Sequence similarities
Belongs to the amidinotransferase family. -
Domain
One chain folds into a compact single domain composed of repeating units, five beta-beta-alpha-beta modules, which surround the central active site. -
Cellular localization
Mitochondrion inner membrane. Cytoplasm. The mitochondrial form is found in the intermembrane space probably attached to the outer side of the inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 2628 Human
- Omim: 602360 Human
- SwissProt: P50440 Human
- Unigene: 75335 Human
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Alternative names
- AGAT antibody
- AT antibody
- GATM antibody
see all
Images
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All lanes : Anti-GATM antibody [OTI1C9] (ab119269) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs
Lane 2 : HEK-293T lysate transfected with pCMV6-ENTRY GATM cDNA for 48 hrs
Lysates/proteins at 5 µg per lane.
Predicted band size: 48 kDa -
Paraffin-embedded human kidney tissue stained for GATM using ab119269 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human liver tissue stained for GATM using ab119269 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human liver carcinoma tissue stained for GATM using ab119269 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human pancreas tissue stained for GATM using ab119269 at 1/100 dilution in immunohistochemical analysis.
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COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY GATM, stained for GATM (Green) using ab119269 at 1/100 dilution in ICC/IF.
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Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with GATM (Red), or an empty vector control plasmid (Blue), stained with ab119269 at 1/100 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab119269 has been referenced in 1 publication.
- Phulukdaree A et al. Atorvastatin increases miR-124a expression: a mechanism of Gamt modulation in liver cells. J Cell Biochem 116:2620-7 (2015). PubMed: 25926069