Overview
Product nameGATM protein (His tag)See all GATM proteins and peptides ...
Protein descriptionRecombinant full length mature Human GATM (amino acids 38-423) fused to N-terminal His tag, 410 amino acids with a predicted MWt of 46.9 kDa including tag (UniProt P50440).
Uniprot accessionP50440
Molecular weight46.900kDa inclusive of tags
Protein length386 amino acids
Expression hostE. coli
Properties
Purity> 90
% by SDS-PAGE
Purification notesab123149 was purified using conventional chromatography.
Mass spectrometryMALDI-TOF
E.C. Number2.1.4.1
FormLiquid
Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 8.00
Constituents: 0.03% DTT, 0.32% Tris HCl, 10% Glycerol, 1.17% Sodium chloride
Concentration information loading...
Sequence notesMGSSHHHHHHSSGLVPRGSHMGSMSTQAATASSRNSCAAD DKATEPLPKDCPVSSYNEWDPLEEVIVGRAENACVPPFTI EVKANTYEKYWPFYQKQGGHYFPKDHLKKAVAEIEEMCNI LKTEGVTVRRPDPIDWSLKYKTPDFESTGLYSAMPRDILI VVGNEIIEAPMAWRSRFFEYRAYRSIIKDYFHRGAKWTTA PKPTMADELYNQDYPIHSVEDRHKLAAQGKFVTTEFEPCF DAADFIRAGRDIFAQRSQVTNYLGIEWMRRHLAPDYRVHI ISFKDPNPMHIDATFNIIGPGIVLSNPDRPCHQIDLFKKA GWTIITPPTPIIPDDHPLWMSSKWLSMNVLMLDEKRVMVD ANEVPIQKMFEKLGITTIKVNIRNANSLGGGFHCWTCDVR RRGTLQSYLD
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab123149
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent concentration. |
| MS |
MS: Use at an assay dependent concentration. |
Application notesThis peptide can be used with studies using ab156265.
Protein info
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Alternative names
GATM_HUMANGlycine amidinotransferaseGlycine amidinotransferase, mitochondrialL-arginine:glycine amidinotransferasemitochondrialTransamidinase
see all
FunctionCatalyzes the biosynthesis of guanidinoacetate, the immediate precursor of creatine. Creatine plays a vital role in energy metabolism in muscle tissues. May play a role in embryonic and central nervous system development. May be involved in the response to heart failure by elevating local creatine synthesis.
Tissue specificityExpressed in brain, heart, kidney, liver, lung, salivary gland and skeletal muscle tissue, with the highest expression in kidney. Biallelically expressed in placenta and fetal tissues.
PathwayAmine and polyamine biosynthesis; creatine biosynthesis; creatine from L-arginine and glycine: step 1/2.
Involvement in diseaseDefects in GATM are the cause of arginine:glycine amidinotransferase deficiency (AGAT deficiency) [MIM:612718]. AGAT deficiency is an autosomal recessive disorder characterized by developmental delay/regression, mental retardation, severe disturbance of expressive and cognitive speech, and severe depletion of creatine/phosphocreatine in the brain.
Sequence similaritiesBelongs to the amidinotransferase family.
DomainOne chain folds into a compact single domain composed of repeating units, five beta-beta-alpha-beta modules, which surround the central active site.
Cellular localizationMitochondrion inner membrane. Cytoplasm. The mitochondrial form is found in the intermembrane space probably attached to the outer side of the inner membrane.
GATM protein (His tag) images
References for GATM protein (His tag) (ab123149)
ab123149
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
SDS-PAGE - GATM protein (ab123149)15% SDS PAGE, 3 µg of ab123149 loaded.