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Anti-GCDFP 15 antibody [23A3] (ab1319)

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Overview

Product name

Anti-GCDFP 15 antibody [23A3]
See all GCDFP 15 products (9) ...

Description

Mouse monoclonal [23A3] to GCDFP 15

Specificity

This antibody is specific to a 15 kD monomer protein called Gross Cystic Disease Fluid Protein 15 (GCDFP 15). Gross cystic disease of the breast is benign premenopausal disorder in which cysts are predominant. These cysts appear to be formed from excessive apocrine cystic secretions, one of which is GCDFP-15. This antibody can be used in for the staining of breast carcinoma, salivary duct carcinoma and apocrine epithelia.

Tested applications

IHC-Fr, IHC-Pmore details

Cross reactivity

Reacts with

Rat, Human

Immunogen

Recombinant protein encoding the excreted domain of human GCDFP-15.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, Tissue culture supernatant

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

23A3

Isotype

IgG2a

Light chain type

kappa

Research areas

Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

  • Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - GCDFP 15 antibody [23A3] (ab1319)Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - GCDFP 15 antibody [23A3] (ab1319) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GCDFP 15 antibody [23A3] (ab1319)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GCDFP 15 antibody [23A3] (ab1319) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab1319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

This antibody may be diluted to a titer of 1:15 to 1:25 in an ABC method.
However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user.
High temperature antigen unmasking with 10mM citrate buffer, pH 6.0 is required prior to immunostaining.
We suggest an incubation period of 60 minutes at room temperature.

Target

Tissue specificity

Expressed in pathological conditions of the mammary gland and in several exocrine tissues, such as the lacrimal, salivary, and sweat glands.

Sequence similarities

Belongs to the PIP family.

Cellular localization

Secreted.

Target information above from: UniProt accessionP12273 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • GCDFP-15 antibody
  • GCDGP15 antibody
  • Gp17 antibody
  • GPIP4 antibody
  • Gross cystic disease fluid protein 15 antibody
  • PIP antibody
  • PIP_HUMAN antibody
  • Prolactin induced protein antibody
  • Prolactin induced protein antibody
  • Prolactin inducible protein precursor antibody
  • Prolactin-induced protein antibody
  • Prolactin-inducible protein antibody
  • SABP antibody
  • Secretory actin binding protein antibody
  • Secretory actin-binding protein antibody
see all

Database links

Anti-GCDFP 15 antibody [23A3] images:

  Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - GCDFP 15 antibody [23A3] (ab1319)

Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - GCDFP 15 antibody [23A3] (ab1319)

Formalin fixed paraffin embedded human skin stained with GCDFP15 using ABC and AEC chromogen.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GCDFP 15 antibody [23A3] (ab1319)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GCDFP 15 antibody [23A3] (ab1319)

ab1319 staining human skin by IHC-P.

References for Anti-GCDFP 15 antibody [23A3] (ab1319)

This product has been referenced in:

  • Hall RE  et al. Prostate-specific antigen and gross cystic disease fluid protein-15 are co-expressed in androgen receptor-positive breast tumours. Br J Cancer 78:360-5 (1998).Read more (PubMed: 9703283) »
  • Viacava P  et al. Spectrum of GCDFP-15 expression in human fetal and adult normal tissues. Virchows Arch 432:255-60 (1998).Read more (PubMed: 9532005) »

See all 2 publications for this product

Publishing research using ab1319? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"