You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-GCDH antibody [3E9BA3BF5] (ab112998)

MSCatalog No. MS781

CodeSizePriceAbpointsAvailability
    
Add to basket
 

  • -

  •   
  •   
  •   

  •  

  •  
  •  

  •  

  •  
Updating...

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »

Overview

Product name

Anti-GCDH antibody [3E9BA3BF5]
See all GCDH products (4) ...

Description

Mouse monoclonal [3E9BA3BF5] to GCDH

Tested applications

ICC/IF, IP, In-Cell ELISA, Flow Cytmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Purified recombinant full length Human GCDH expressed in E.coli.

Positive control

Fibroblast, HeLa, HepG2, and Rat Cardiomyocyte cells.

Properties

Form

Liquid

Storage instructions

Store at 4°C or at -20°C for long term storage.

Storage buffer

Preservative: 0.02% Sodium azide
Constituent: 99.98% HBS

Concentration

Concentration information loading...

Purity

>95% by SDS-PAGE

Purification notes

ab112998 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE.

Clonality

Monoclonal

Clone number

3E9BA3BF5

Isotype

IgG1

Light chain type

kappa

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Amino acid metabolism
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Amino Acids
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria

  • Immunocytochemistry/ Immunofluorescence - Anti-GCDH antibody [3E9BA3BF5] (ab112998)Immunocytochemistry/ Immunofluorescence - Anti-GCDH antibody [3E9BA3BF5] (ab112998) image (enlarge)

  • Flow Cytometry - GCDH antibody [3E9BA3BF5] (ab112998)Flow Cytometry - GCDH antibody [3E9BA3BF5] (ab112998) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab112998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. Isoform Short is inactive.

Tissue specificity

Isoform 1 and isoform 2 are expressed in fibroblasts and liver.

Pathway

Amino-acid metabolism; lysine degradation.
Amino-acid metabolism; tryptophan metabolism.

Involvement in disease

Defects in GCDH are the cause of glutaric aciduria type 1 (GA1) [MIM:231670]. GA1 is an autosomal recessive metabolic disorder characterized by progressive dystonia and athetosis due to gliosis and neuronal loss in the basal ganglia.

Sequence similarities

Belongs to the acyl-CoA dehydrogenase family.

Cellular localization

Mitochondrion matrix.

Target information above from: UniProt accessionQ92947 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • mitochondrial antibody
  • ACAD5 antibody
  • EC 1.3.99.7 antibody
  • GCD antibody
  • Gcdh antibody
  • GCDH_HUMAN antibody
  • Glutaryl CoA dehydrogenase, mitochondrial antibody
  • Glutaryl Coenzyme A dehydrogenase antibody
  • Glutaryl-CoA dehydrogenase antibody
  • MS781 antibody
see all

Database links

see all

Anti-GCDH antibody [3E9BA3BF5] images:

  Immunocytochemistry/ Immunofluorescence - Anti-GCDH antibody [3E9BA3BF5] (ab112998)

Immunocytochemistry/ Immunofluorescence - Anti-GCDH antibody [3E9BA3BF5] (ab112998)

ab112998 at 1 µg/ml staining GCDH in fibroblast cells by immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). Nuclei were stained with DAPI.

  Flow Cytometry - GCDH antibody [3E9BA3BF5] (ab112998)

Flow Cytometry - GCDH antibody [3E9BA3BF5] (ab112998)

ab112998 at 1ug/ml staining GCDH in HeLa cells by Flow Cytometry (blue). Isotype control antibody (red).

References for Anti-GCDH antibody [3E9BA3BF5] (ab112998)

ab112998 has not yet been referenced specifically in any publications.

Publishing research using ab112998? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"