Overview

  • Product name
    Anti-GCDH antibody [3E9BA3BF5]
    See all GCDH primary antibodies
  • Description
    Mouse monoclonal [3E9BA3BF5] to GCDH
  • Tested applications
    Suitable for: ICC/IF, IP, In-Cell ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Purified recombinant full length Human GCDH expressed in E.coli.

  • Positive control
    • Fibroblast, HeLa, HepG2, and Rat Cardiomyocyte cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab112998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IP Use at an assay dependent concentration.
In-Cell ELISA Use a concentration of 1 µg/ml. 0.1 µg/well.
Flow Cyt Use a concentration of 1 µg/ml. 0.1% Triton X-100 permeabilization recommended.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. Isoform Short is inactive.
  • Tissue specificity
    Isoform 1 and isoform 2 are expressed in fibroblasts and liver.
  • Pathway
    Amino-acid metabolism; lysine degradation.
    Amino-acid metabolism; tryptophan metabolism.
  • Involvement in disease
    Defects in GCDH are the cause of glutaric aciduria type 1 (GA1) [MIM:231670]. GA1 is an autosomal recessive metabolic disorder characterized by progressive dystonia and athetosis due to gliosis and neuronal loss in the basal ganglia.
  • Sequence similarities
    Belongs to the acyl-CoA dehydrogenase family.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACAD5 antibody
    • EC 1.3.99.7 antibody
    • GCD antibody
    • Gcdh antibody
    • GCDH_HUMAN antibody
    • Glutaryl CoA dehydrogenase antibody
    • Glutaryl CoA dehydrogenase, mitochondrial antibody
    • Glutaryl Coenzyme A dehydrogenase antibody
    • Glutaryl-CoA dehydrogenase antibody
    • mitochondrial antibody
    • MS781 antibody
    see all

Images

  • ab112998 at 1 µg/ml staining GCDH in fibroblast cells by immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). Nuclei were stained with DAPI.
  • ab112998 at 1ug/ml staining GCDH in HeLa cells by Flow Cytometry (blue). Isotype control antibody (red).

References

ab112998 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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