Anti-GCET1 antibody [RAM341] (ab68889)
Key features and details
- Mouse monoclonal [RAM341] to GCET1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-GCET1 antibody [RAM341]
See all GCET1 primary antibodies -
Description
Mouse monoclonal [RAM341] to GCET1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: IHC-Fr -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human GCET1.
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Positive control
- IHC-P: Human Burkitt's Lymphoma, Follicular B Cell Lymphoma, Diffuse Large B Cell Lymphoma and Lymphocyte Predominant Hodgkin's Lymphoma tissue. Human tonsil tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
This antibody was purified using a AKTA prime. -
Clonality
Monoclonal -
Clone number
RAM341 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100.
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Notes |
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IHC-P
1/100. |
Target
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Function
Protease inhibitor that inhibits trypsin and trypsin-like serine proteases (in vitro). Inhibits plasmin and thrombin with lower efficiency (in vitro). -
Tissue specificity
Highly expressed in normal germinal center (GC) B-cells and GC B-cell-derived malignancies. -
Sequence similarities
Belongs to the serpin family. -
Cellular localization
Cytoplasm; Secreted and Membrane. - Information by UniProt
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Database links
- Entrez Gene: 327657 Human
- SwissProt: Q86WD7 Human
- Unigene: 317970 Human
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Alternative names
- Centerin antibody
- GCET1 antibody
- Germinal center B cell expressed transcript 1 antibody
see all
Images
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Ab68889 staining human GCET1 in Burkitt's Lymphoma (A), Follicular B Cell Lymphoma (B), Diffuse Large B Cell Lymphoma (C) and Lymphocyte Predominant Hodgkin's Lymphoma (D) by immunohistochemistry using formalin fixed, paraffin embedded tissue.
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IHC image of GCET1 staining in human tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68889, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Ab68889 staining human GCET1 in human tonsil by immunohistochemistry using paraffin embedded tissue.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab68889 has been referenced in 10 publications.
- Perfecto-Avalos Y et al. Discriminant analysis and machine learning approach for evaluating and improving the performance of immunohistochemical algorithms for COO classification of DLBCL. J Transl Med 17:198 (2019). PubMed: 31185999
- Tessier-Cloutier B et al. Cell of origin in diffuse large B-cell lymphoma in systemic lupus erythematosus: molecular and clinical factors associated with survival. Lupus Sci Med 6:e000324 (2019). PubMed: 31205728
- Boltežar L et al. Comparison of the algorithms classifying the ABC and GCB subtypes in diffuse large B-cell lymphoma. Oncol Lett 15:6903-6912 (2018). PubMed: 29731865
- Lu TX et al. Epstein-Barr virus positive diffuse large B-cell lymphoma predict poor outcome, regardless of the age. Sci Rep 5:12168 (2015). IHC ; Human . PubMed: 26202875
- Tzankov A et al. Multiparameter analysis of homogeneously R-CHOP-treated diffuse large B cell lymphomas identifies CD5 and FOXP1 as relevant prognostic biomarkers: report of the prospective SAKK 38/07 study. J Hematol Oncol 8:70 (2015). IHC . PubMed: 26071053
- Xie Y et al. p53 expression is a strong marker of inferior survival in de novo diffuse large B-cell lymphoma and may have enhanced negative effect with MYC coexpression: a single institutional clinicopathologic study. Am J Clin Pathol 141:593-604 (2014). Human . PubMed: 24619762
- Tan LH & Tan SY Aberrant immunoarchitecture distinguishes hyperplastic germinal centres in pattern 1 angioimmunoblastic T-cell lymphoma from reactive follicles. Hematol Oncol 32:145-54 (2014). Human . PubMed: 24254640
- Rossi D et al. Molecular history of Richter syndrome: origin from a cell already present at the time of chronic lymphocytic leukemia diagnosis. Int J Cancer 130:3006-10 (2012). PubMed: 21796624
- Sebestyén A et al. Activity and complexes of mTOR in diffuse large B-cell lymphomas-a tissue microarray study. Mod Pathol : (2012). PubMed: 22899290
- Montes-Moreno S et al. Gcet1 (centerin), a highly restricted marker for a subset of germinal center-derived lymphomas. Blood 111:351-8 (2008). PubMed: 17898315