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Products:Signal Transduction >> Metabolism >> Mitochondrial
Anti-GCLC antibody - BSA and Azide free (ab80841)
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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab80841? Please let us know so that we can cite the reference in this datasheet
ab80841 has been referenced in 1 publications.
- Wang X et al. Activation of the Nuclear Factor E2-Related Factor 2/Antioxidant Response Element Pathway is Neuroprotective Following Spinal Cord Injury. J Neurotrauma : (2011). WB; Rat. PubMed: 21806470
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - GCLC antibody - BSA and Azide free (ab80841)
Anti-GCLC antibody - BSA and Azide free (ab80841) at 2.5 µg/ml
Predicted band size : 73 kDa
Observed band size : 73 kDa
This image is produced using the version of this antibody, containing BSA and azide.
Immunocytochemistry/ Immunofluorescence - Anti-GCLC antibody - BSA and Azide free (ab80841)
ICC/IF image of ab80841 stained PC12 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80841, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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