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Products:Signal Transduction >> Metabolism >> Mitochondrial
Anti-GCLC antibody - BSA and Azide free (ab80841)
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Western blot - GCLC antibody - BSA and Azide free (ab80841)
Anti-GCLC antibody - BSA and Azide free (ab80841) at 2.5 µg/ml
Predicted band size : 73 kDa
Observed band size : 73 kDa
This image is produced using the version of this antibody, containing BSA and azide.
Immunocytochemistry/ Immunofluorescence - Anti-GCLC antibody - BSA and Azide free (ab80841)
ICC/IF image of ab80841 stained PC12 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80841, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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