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Products:Signal Transduction >> Metabolism >> Mitochondrial
Overview
Product name
Anti-GCLC antibody - BSA and Azide free
See all GCLC products (6) ...
Description
Rabbit polyclonal to GCLC - BSA and Azide free
Specificity
ab80841 recognizes the heavy subunit of GCLC.
Tested applications
WB, IP, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human, Monkey
Predicted to work with
Xenopus laevis, Zebrafish, Cynomolgus Monkey
Immunogen
Synthetic peptide: CRWGVISASVDDRTREERG, corresponding to internal sequence amino acids 295 - 313 of Rat GCLC.
CRWGVISASV DDRTREERG
Epitope
Amino acids 295-313.
Positive control
Jurkat, MAD109 and PC12 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Other Antibodies
Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Mitochondrial
Applications
Show applications key
Our Abpromise guarantee covers the use of ab80841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2.5 - 5 µg/ml.Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
IP: Use at 10 µg/mg of lysate. ((Denatured, use Protein A).)
ICC/IF: Use a concentration of 1 µg/ml
Target
Pathway
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
Involvement in disease
Defects in GCLC are the cause of hemolytic anemia (HAGGSD) [MIM:230450].
Sequence similarities
Belongs to the glutamate--cysteine ligase type 3 family.
Target information above from: UniProt accessionP48506
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 6.3.2.2 antibody
- Gamma ECS antibody
- Gamma glutamylcysteine synthetase antibody
see all
Database links
- Entrez Gene: 2729 Human
- Entrez Gene: 14629 Mouse
- Entrez Gene: 25283 Rat
- Entrez Gene: 326857 Zebrafish
- Omim: 606857 Human
- SwissProt: P48506 Human
- SwissProt: P97494 Mouse
- SwissProt: P19468 Rat
see all
Anti-GCLC antibody - BSA and Azide free images:
Western blot - GCLC antibody - BSA and Azide free (ab80841)
Anti-GCLC antibody - BSA and Azide free (ab80841) at 2.5 µg/ml
Predicted band size : 73 kDa
Observed band size : 73 kDa
This image is produced using the version of this antibody, containing BSA and azide.
Immunocytochemistry/ Immunofluorescence - Anti-GCLC antibody - BSA and Azide free (ab80841)
ICC/IF image of ab80841 stained PC12 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80841, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-GCLC antibody - BSA and Azide free (ab80841)
This product has been referenced in:
- Wang Xet al. Activation of the Nuclear Factor E2-Related Factor 2/Antioxidant Response Element Pathway is Neuroprotective Following Spinal Cord Injury. J Neurotrauma : (2011). WB; Rat.Read more (PubMed: 21806470) »
See 1 publication for this product
Publishing research using ab80841? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"