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Products:Signal Transduction >> Metabolism >> Mitochondrial
Overview
Product name
Anti-GCLC antibody
See all GCLC products (6) ...
Description
Rabbit polyclonal to GCLC
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human GCLC.
(Peptide available as ab41462.)
Positive control
This antibody gave a positive signal in the following Human Tissue Lysates: Liver, Kidney and Lung (Adenocarcinoma).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Other Antibodies
Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Mitochondrial
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Western blot - GCLC antibody (ab41463)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab41463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
Target
Pathway
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
Involvement in disease
Defects in GCLC are the cause of hemolytic anemia (HAGGSD) [MIM:230450].
Sequence similarities
Belongs to the glutamate--cysteine ligase type 3 family.
Target information above from: UniProt accessionP48506
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 6.3.2.2 antibody
- Gamma ECS antibody
- Gamma GCS antibody
see all
Database links
- Entrez Gene: 2729 Human
- Entrez Gene: 14629 Mouse
- Entrez Gene: 25283 Rat
- Omim: 606857 Human
- SwissProt: P48506 Human
- SwissProt: P97494 Mouse
- SwissProt: P19468 Rat
- Unigene: 654465 Human
see all
Anti-GCLC antibody images:
Western blot - GCLC antibody (ab41463)
All lanes : Anti-GCLC antibody (ab41463) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Lung (Human) Tissue Lysate - Adenocarcinoma (ab42923)
Lane 3 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)
Lane 4 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 73 kDa
Observed band size : 73 kDa
Additional bands at : 58 kDa,60 kDa. We are unsure as to the identity of these extra bands.
References for Anti-GCLC antibody (ab41463)
This product has been referenced in:
- Ungvari Zet al. Adaptive induction of NF-E2-related factor-2-driven antioxidant genes in endothelial cells in response to hyperglycemia. Am J Physiol Heart Circ Physiol 300:H1133-40 (2011). WB; Mouse.Read more (PubMed: 21217061) »
- Ungvari Zet al. Vascular oxidative stress in aging: a homeostatic failure due to dysregulation of NRF2-mediated antioxidant response. Am J Physiol Heart Circ Physiol 301:H363-72 (2011). WB; Rat.Read more (PubMed: 21602469) »
See all 4 publications for this product
Publishing research using ab41463? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"