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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab53179? Please let us know so that we can cite the reference in this datasheet
ab53179 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunocytochemistry/ Immunofluorescence - GCLC antibody (ab53179)
ab53179 staining mouse embryo cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton-X prior to blocking with 5% serum for 1 hr at 25°C. The primary antibody was diluted 1/500 in PBS with 0.5% goat serum and incubated with the sample for 18 hrs at 4°C. A FITC-conjugated goat anti-rabbit was used as the secondary antibody. Note spindle staining in cells undergoing mitosis
This image is courtesy of an anonymous Abreview
Western blot - GCLC antibody (ab53179)
Anti-GCLC antibody (ab53179) at 1/25 dilution + lysate prepared from kidney
Gamma Glutamylcysteine Synthetase / GCS antibody for ICC/IF in Mouse (53179)
GCLC antibody for Western blot in Mouse (53179)
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