Anti-GCLC antibody (ab53179)

Would we be able to have a replacement antibody? But instead of the ab53179, could we replace it with an ab41463 please? As I feel this would be more suited to our experiments. If you could help with this it would be highly appreciated.   Thank you,  

ANSWER:

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab41463. Currently ab41463 is backordered. You should expect to receive this shipment around Dec. 6. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

LOT NUMBER GR40310-2 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Multiple bands -Strong bands around 45KDa and 95KDa, GCLC band at 73KDa is very weak SAMPLE Mouse Liver lysates PRIMARY ANTIBODY Abcam ab53179 - Multiple concentrations used from 1:5000 to 1:20000, 1hour-4hour incubations, up to 1 hour of washing. DETECTION METHOD Licor Odessey ANTIBODY STORAGE CONDITIONS -20 freezer SAMPLE PREPARATION laemmli (50%), diluted in dH20. Samples heated to 80degrees for 10mins. AMOUNT OF PROTEIN LOADED 20ug/10ul ELECTROPHORESIS/GEL CONDITIONS 10% gel TRANSFER AND BLOCKING CONDITIONS We have used both 10% Milk and 10% BSA, overnight and for 1hour SECONDARY ANTIBODY IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/20000 dilution HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 10 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Primary concentration, development method (ECLplus to Odessey), Incubation times, Wash times, Blocking buffer (BSA to Milk)

ANSWER:

Thank you for submitting your completed complaint questionnaire. It does appear that this antibody is not detecting GCLC as expected. If you have purchased this antibody within the last 6 months or so I would be happy to offer you a free replacement, credit or refund. In your reply, please include the purchase order number or Abcam order reference number associated with the initial order of ab53179 so I can process your request. Thanks! I look forward to hearing from you.