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Products:Signal Transduction >> Metabolism >> Mitochondrial
Overview
Product name
Anti-GCLC antibody
See all GCLC products (6) ...
Description
Rabbit polyclonal to GCLC
Tested applications
ICC/IF, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat
Immunogen
Synthetic peptide corresponding to internal region of rat GCLC protein
Positive control
Kidney and Testis
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Other Antibodies
Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Mitochondrial
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
IHC-P: 1/100Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at an assay dependent dilution.
Target
Pathway
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
Involvement in disease
Defects in GCLC are the cause of hemolytic anemia (HAGGSD) [MIM:230450].
Sequence similarities
Belongs to the glutamate--cysteine ligase type 3 family.
Target information above from: UniProt accessionP48506
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 6.3.2.2 antibody
- Gamma ECS antibody
- Gamma GCS antibody
see all
Database links
- Entrez Gene: 2729 Human
- Entrez Gene: 14629 Mouse
- Entrez Gene: 25283 Rat
- Omim: 606857 Human
- SwissProt: P48506 Human
- SwissProt: P97494 Mouse
- SwissProt: P19468 Rat
- Unigene: 654465 Human
see all
Anti-GCLC antibody images:
Immunocytochemistry/ Immunofluorescence - GCLC antibody (ab53179)
ab53179 staining mouse embryo cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton-X prior to blocking with 5% serum for 1 hr at 25°C. The primary antibody was diluted 1/500 in PBS with 0.5% goat serum and incubated with the sample for 18 hrs at 4°C. A FITC-conjugated goat anti-rabbit was used as the secondary antibody. Note spindle staining in cells undergoing mitosis
This image is courtesy of an anonymous Abreview
Western blot - GCLC antibody (ab53179)
Anti-GCLC antibody (ab53179) at 1/25 dilution + lysate prepared from kidney
References for Anti-GCLC antibody (ab53179)
ab53179 has not yet been referenced specifically in any publications.
Publishing research using ab53179? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"