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Products:Signal Transduction >> Metabolism >> Mitochondrial
Overview
Product name
Anti-GCLC antibody
See all GCLC products (6) ...
Description
Mouse monoclonal to GCLC
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment, corresponding to amino acids 528-638 of Human GCLC.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Cell Biology >> Other Antibodies >> Other Antibodies
Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Mitochondrial
Applications
Show applications key
Our Abpromise guarantee covers the use of ab55435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 73 kDa.
ICC/IF: Use a concentration of 10 µg/ml
Target
Pathway
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
Involvement in disease
Defects in GCLC are the cause of hemolytic anemia (HAGGSD) [MIM:230450].
Sequence similarities
Belongs to the glutamate--cysteine ligase type 3 family.
Target information above from: UniProt accessionP48506
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 6.3.2.2 antibody
- Gamma ECS antibody
- Gamma glutamylcysteine synthetase antibody
see all
Database links
- Entrez Gene: 2729 Human
- Omim: 606857 Human
- SwissProt: P48506 Human
- Unigene: 654465 Human
Anti-GCLC antibody images:
Western blot - GCLC antibody (ab55435)
Predicted band size : 73 kDa
GCLC (ab55435) at 1ug/lane + A-431 cell lysate at 25ug/lane.
Immunocytochemistry/ Immunofluorescence-GCLC antibody(ab55435)
ab55435 at 10 ug/ml staining GCLC in human Hella cells by Immunocytochemistry/ Immunofluorescence.
References for Anti-GCLC antibody (ab55435)
ab55435 has not yet been referenced specifically in any publications.
Publishing research using ab55435? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"