Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
Overview
Product name
Anti-GCLM antibody
See all GCLM products (3) ...
Description
Rabbit polyclonal to GCLM
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow
Immunogen
A synthetic peptide corresponding to a region within internal amino acids 180-229 QVKPNSNQVN LASCCVMPPD LTAFAKQFDI QLLTHNDPKE LLSEASFQEA of Human GCLM.
QVKPNSNQVN LASCCVMPPD LTAFAKQFDI QLLTHNDPKE LLSEASFQEA
Positive control
MCF7 Cell Lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Drug metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
Signal Transduction >> Metabolism >> Amino Acids
Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
-
Western blot - GCLM antibody (ab81445)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab81445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 31 kDa.
Target
Tissue specificity
In all tissues examined. Highest levels in skeletal muscle.
Pathway
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
Sequence similarities
Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.
Target information above from: UniProt accessionP48507
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Gamma ECS regulatory subunit antibody
- Gamma-ECS regulatory subunit antibody
- Gamma-glutamylcysteine synthetase regulatory subunit antibody
see all
Database links
- Entrez Gene: 2730 Human
- Entrez Gene: 14630 Mouse
- Entrez Gene: 29739 Rat
- SwissProt: P48507 Human
- SwissProt: O09172 Mouse
- SwissProt: P48508 Rat
- Unigene: 315562 Human
- Unigene: 292676 Mouse
see all
Anti-GCLM antibody images:
Western blot - GCLM antibody (ab81445)
Anti-GCLM antibody (ab81445) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 Cell Lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 31 kDa
Observed band size : 31 kDa
Gel Concentration: 12%
References for Anti-GCLM antibody (ab81445)
ab81445 has not yet been referenced specifically in any publications.
Publishing research using ab81445? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"