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Western blot - GCM2 antibody (ab64723)
Western blot - GCM2 antibody (ab64723)
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Product Name 

GCM2 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to GCM2

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus GCM2.

(Peptide available as ab69686.)

Reacts with 
(species key)

Ms, Rat

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

This antibody gave a positive signal in the following lysates:
F9 whole cell
Brain (Mouse) tissue
Brain (Mouse) tissue, 0 days old
Skeletal Muscle (Mouse) Tissue
Thymus (Rat) Tissue

Cellular localization 

Nuclear

Research areas 

Developmental Biology >> Lineage specification >> Ectoderm
Stem Cells >> Lineage Markers >> Ectoderm

Relevance 

Gcm2, a mouse ortholog of the Drosophila Glial Cells Missing gene, is expressed in the parathyroid-specific domains in the 3rd pouches from E9.5. The null mutation of Gcm2 causes aparathyroidism in the fetal and adult mouse and has been proposed to be a master regulator for parathyroid development. During Drosophila embryogenesis Gcm2 plays a crucial role in promoting glial cell differentiation.

 

Alternative names GCM2 antibody (ab64723)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

107889    (Mouse)

SwissProt

    

O09102    (Mouse)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 0.85 - 0.85 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GCM2 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for GCM2 antibody images, references, products related to ab64723 and other tools.

 

GCM2 antibody images:

Product image  Western blot - GCM2 antibody (ab64723)

Western blot - GCM2 antibody (ab64723)

All lanes : GCM2 antibody (ab64723) at 1 µg/ml

Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188)
Lane 4 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 5 : Thymus (Rat) Tissue Lysate
Lane 6 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate with GCM2 peptide (ab69686) at 1 µg/ml
Lane 7 : Brain (Mouse) Tissue Lysate with GCM2 peptide (ab69686) at 1 µg/ml
Lane 8 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) with GCM2 peptide (ab69686) at 1 µg/ml
Lane 9 : Skeletal Muscle (Mouse) Tissue Lysate with GCM2 peptide (ab69686) at 1 µg/ml
Lane 10 : Thymus (Rat) Tissue Lysate with GCM2 peptide (ab69686) at 1 µg/ml

Lysates/proteins at 10 µg/ml per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 56 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 2 minutes

 
 

Search PubMed (MEDLINE) for references to GCM2

 

GCM2 antibody - more information

Customer reviews (feedback) regarding GCM2 antibody

Customer FAQs regarding GCM2 antibody

Protocols for GCM2 antibody

Price and availability of products related to GCM2 antibody

 

GCM2 antibody - related products:

Products for Western blot

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Lysates

 

Immunizing Peptide for GCM2 antibody

ab69686

  

GCM2 peptide

 

Compatible Secondaries for GCM2 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

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GCM2 antibody - other tools:

Contact Abcam with a Technical Enquiry about GCM2 antibody

 

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