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Products:Stem Cells >> Lineage Markers >> Ectoderm
Overview
Product name
GCM2 peptide
Protein description
Synthetic peptide derived from within residues 450 to the C-terminus of Human GCM2.(Note: the amino acid sequence is proprietary)This peptide was used as an immunogen for ab64723 - GCM2 antibody.
Protein length
504 amino acids
Properties
Purity
70 - 90% by HPLC
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Information available upon request.
Concentration
Concentration information loading...
Research areas
Developmental Biology >> Lineage specification >> Ectoderm
Stem Cells >> Lineage Markers >> Ectoderm
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Western blot - GCM2 antibody (ab64723)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab69686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking
Application notes
BL: Use at a concentration of 1 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Relevance
Gcm2, a mouse ortholog of the Drosophila Glial Cells Missing gene, is expressed in the parathyroid-specific domains in the 3rd pouches from E9.5. The null mutation of Gcm2 causes aparathyroidism in the fetal and adult mouse and has been proposed to be a master regulator for parathyroid development. During Drosophila embryogenesis Gcm2 plays a crucial role in promoting glial cell differentiation.
Cellular localization
Nuclear
GCM2 peptide images:
Western blot - GCM2 antibody (ab64723)
All lanes : Anti-GCM2 antibody (ab64723) at 1 µg/ml
Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188)
Lane 4 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 5 : Thymus (Rat) Tissue Lysate
Lane 6 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate with GCM2 peptide (ab69686) at 1 µg/ml
Lane 7 : Brain (Mouse) Tissue Lysate with GCM2 peptide (ab69686) at 1 µg/ml
Lane 8 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) with GCM2 peptide (ab69686) at 1 µg/ml
Lane 9 : Skeletal Muscle (Mouse) Tissue Lysate with GCM2 peptide (ab69686) at 1 µg/ml
Lane 10 : Thymus (Rat) Tissue Lysate with GCM2 peptide (ab69686) at 1 µg/ml
Lysates/proteins at 10 µg/ml per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 56 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
References for GCM2 peptide (ab69686)
ab69686 has not yet been referenced specifically in any publications.
Publishing research using ab69686? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"