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Western blot - GCM2 antibody (ab64723)
Western blot - GCM2 antibody (ab64723)
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Product Name 

GCM2 peptide

Product type 

Proteins and Peptides

Peptide 

Synthetic peptide derived from within residues 450 to the C-terminus of Human GCM2.This peptide was used as an immunogen for ab64723 - GCM2 antibody.

Tested applications 
(see key)

BL

Application notes 
(see key)

Recommended dilutions
BL: Use at a concentration of 1 µg/ml.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Developmental Biology >> Lineage specification >> Ectoderm
Stem Cells >> Lineage Markers >> Ectoderm

Relevance 

Gcm2, a mouse ortholog of the Drosophila Glial Cells Missing gene, is expressed in the parathyroid-specific domains in the 3rd pouches from E9.5. The null mutation of Gcm2 causes aparathyroidism in the fetal and adult mouse and has been proposed to be a master regulator for parathyroid development. During Drosophila embryogenesis Gcm2 plays a crucial role in promoting glial cell differentiation.

 

Alternative names GCM2 peptide (ab69686)

Purity 

70 - 90% by HPLC

Storage buffer 

Preservative: None
Constituents: 0.001% Tween 20, 30mM HEPES, 2mM EDTA, 150mM Sodium chloride, pH 6.75

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GCM2 peptide is on this datasheet. But please do contact us if you would like any reassurance!


See below for GCM2 peptide images, references, products related to ab69686 and other tools.

 

GCM2 peptide images:

Product image  Western blot - GCM2 antibody (ab64723)

Western blot - GCM2 antibody (ab64723)

All lanes : GCM2 antibody (ab64723) at 1 µg/ml

Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188)
Lane 4 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 5 : Thymus (Rat) Tissue Lysate
Lane 6 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate with GCM2 peptide (ab69686) at 1 µg/ml
Lane 7 : Brain (Mouse) Tissue Lysate with GCM2 peptide (ab69686) at 1 µg/ml
Lane 8 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) with GCM2 peptide (ab69686) at 1 µg/ml
Lane 9 : Skeletal Muscle (Mouse) Tissue Lysate with GCM2 peptide (ab69686) at 1 µg/ml
Lane 10 : Thymus (Rat) Tissue Lysate with GCM2 peptide (ab69686) at 1 µg/ml

Lysates/proteins at 10 µg/ml per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 56 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 2 minutes

 
 

Search PubMed (MEDLINE) for references to GCM2

 

GCM2 peptide - more information

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Price and availability of products related to GCM2 peptide

 

GCM2 peptide - related products:

 

Corresponding Antibody for GCM2 peptide

ab64723

  

Rabbit polyclonal to GCM2

 

Other GCM2 Antibodies

See all GCM2 antibodies (1)...

 

GCM2 peptide - other tools:

Contact Abcam with a Technical Enquiry about GCM2 peptide

 

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