Feedback Form

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GCN2 antibody (ab37674)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GCN2 antibody (ab37674)
(enlarge)


Western blot - GCN2 antibody (ab37674)
Western blot - GCN2 antibody (ab37674)
(enlarge)


Product Name 

GCN2 antibody

 

See all GCN2 antibodies (4)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to GCN2

Immunogen 

Synthetic peptide selected from the center region with phenylalanine at position 1116 of human GCN2.

Reacts with 
(species key)

Hu, Ms

Tested applications 
(see key)

ELISA, IHC-P, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews

Submit an Abreview

Be the first to review this antibody and earn 220 Abpoints.


Application notes 
(see key)

Recommended dilutions
ELISA: 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Predicted molecular weight: 187 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

293 cell lysate.
NIH 3T3 cell lysate.
Human breast carcinoma tissue.

Cellular localization 

Cytoplasmic

Research areas 

Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation

Relevance 

GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses.

 

Alternative names GCN2 antibody (ab37674)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

440275    (Human)
27103    (Mouse)

GeneCard

    

GC15P038014    (Human)

Omim

    

609280    (Human)

SwissProt

    

Q9P2K8    (Human)
Q9QZ05    (Mouse)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein G purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.250 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GCN2 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for GCN2 antibody images, references, products related to ab37674 and other tools.

 

GCN2 antibody images:

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GCN2 antibody (ab37674)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GCN2 antibody (ab37674)

Immunohistochemical analysis of GCN2 expression in paraffin embedded formalin fixed human breast carcinoma with 1/50 ab37674.

 

Product image  Western blot - GCN2 antibody (ab37674)

Western blot - GCN2 antibody (ab37674)

All lanes : GCN2 antibody (ab37674) at 1/500 dilution

Lane 1 : 293 cell lysates non-transfected.
Lane 2 : 293 cell lysates transiently transfected with GCN2 gene.

Lysates/proteins at 2 µg per lane.


Predicted band size : 187 kDa

 
 

Search PubMed (MEDLINE) for references to GCN2

 

GCN2 antibody - more information

Customer reviews (feedback) regarding GCN2 antibody

Customer FAQs regarding GCN2 antibody

Protocols for GCN2 antibody

Price and availability of products related to GCN2 antibody

 

GCN2 antibody - related products:

Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Slides

Products for Western blot

Loading controls

Lysates

 

Positive Controls for GCN2 antibody

ab7179

  

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

 

Compatible Secondaries for GCN2 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other GCN2 Antibodies

See all GCN2 antibodies (4)...

 

GCN2 antibody - other tools:

Contact Abcam with a Technical Enquiry about GCN2 antibody

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.