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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-GCN2 antibody
See all GCN2 products (5) ...
Description
Rabbit polyclonal to GCN2
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide selected from the center region with phenylalanine at position 1116 of human GCN2.
Positive control
293 cell lysate. NIH 3T3 cell lysate. Human breast carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/100 - 1/500.Predicted molecular weight: 187 kDa.
IHC-P: 1/50 - 1/100.
Target
Function
Can phosphorylate the alpha subunit of EIF2 and may mediate translational control.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 protein kinase domains.
Contains 1 RWD domain.
Domain
Kinase domain 1 is a degenerate kinase domain.
RWD domain is reported to interact with GCN1L1.
Post-translational
modifications
Autophosphorylated on threonine residues.
Target information above from: UniProt accessionQ9P2K8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E2AK4_HUMAN antibody
- EIF2AK4 antibody
- Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody
see all
Database links
- Entrez Gene: 440275 Human
- Entrez Gene: 27103 Mouse
- Omim: 609280 Human
- SwissProt: Q9P2K8 Human
- SwissProt: Q9QZ05 Mouse
- Unigene: 656673 Human
- Unigene: 217616 Mouse
Anti-GCN2 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GCN2 antibody (ab37674)
Immunohistochemical analysis of GCN2 expression in paraffin embedded formalin fixed human breast carcinoma with 1/50 ab37674.
Western blot - GCN2 antibody (ab37674)
All lanes : Anti-GCN2 antibody (ab37674) at 1/500 dilution
Lane 1 : 293 cell lysates non-transfected.
Lane 2 : 293 cell lysates transiently transfected with GCN2 gene.
Lysates/proteins at 2 µg per lane.
Predicted band size : 187 kDa
References for Anti-GCN2 antibody (ab37674)
ab37674 has not yet been referenced specifically in any publications.
Publishing research using ab37674? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"