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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab70214? Please let us know so that we can cite the reference in this datasheet
ab70214 has been referenced in 1 publications.
- Kwon NH et al. Dual role of methionyl-tRNA synthetase in the regulation of translation and tumor suppressor activity of aminoacyl-tRNA synthetase-interacting multifunctional protein-3. Proc Natl Acad Sci U S A 108:19635-40 (2011). WB; Human. PubMed: 22106287
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - GCN2 antibody (ab70214)
All lanes : Anti-GCN2 antibody (ab70214) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Predicted band size : 187 kDa
Observed band size : 187 kDa
Additional bands at : 171 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 seconds
Immunoprecipitation - GCN2 antibody (ab70214)
Detection of Human GCN2 by Immunoprecipitation in Whole cell lysate from HeLa cells using ab70214 at 3 µg/mg lysate for IP and at 0.1 µg/ml for subsequent WB detection.
Immunocytochemistry/ Immunofluorescence - Anti-GCN2 antibody (ab70214)
ICC/IF image of ab70214 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70214, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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