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All lanes : Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427) at 1/2000 dilution
Lane 1 : HeLa cell lysate - untreated
Lane 2 : HeLa cell lysate - treated with LP
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution
Predicted band size : 220 kDa
Observed band size : 220 kDa
Additional bands at : 180 kDa. We are unsure as to the identity of these extra bands.
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