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Western blot - GCN2 (phospho T667) antibody [EPR2319Y] (ab68427)
![Western blot - GCN2 (phospho T667) antibody [EPR2319Y] (ab68427)](/ps/datasheet/images/GCN2_WB.gif)
(enlarge)
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Product Name
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GCN2 (phospho T667) antibody [EPR2319Y]
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See all GCN2 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EPR2319Y] to GCN2 (phospho T667)
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Immunogen
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Synthetic phosphopeptide corresponding to residues surrounding threonine 667 of human GCN2.
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Reacts with
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Hu
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Does not react with
(species key)
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Ms, Rat
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Specificity
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Detects GCN2 only when phosphorylyated at threonine 667.
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: 1/30.
WB: 1/1000 - 1/2000. Detects a band of approximately 220 kDa (predicted molecular weight: 220 kDa).
Is unsuitable for Flow Cyt, ICC/IF or IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cells
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
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Relevance
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GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses.
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GCN2 (phospho T667) antibody [EPR2319Y] (ab68427) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EPR2319Y
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 50mM Tris glycine, 0.15mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GCN2 (phospho T667) antibody [EPR2319Y] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GCN2 (phospho T667) antibody [EPR2319Y] images, references, products related to ab68427 and other tools.
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GCN2 (phospho T667) antibody [EPR2319Y] images:
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 Western blot - GCN2 (phospho T667) antibody [EPR2319Y] (ab68427)
All lanes : GCN2 (phospho T667) antibody [EPR2319Y] (ab68427) at 1/2000 dilution
Lane 1 : HeLa cell lysate - untreated
Lane 2 : HeLa cell lysate - treated with LP
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution
Predicted band size : 220 kDa
Observed band size : 220 kDa
Additional bands at : 180 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to GCN2
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GCN2 (phospho T667) antibody [EPR2319Y] - more information
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Customer reviews (feedback) regarding GCN2 (phospho T667) antibody [EPR2319Y] |
Customer FAQs regarding GCN2 (phospho T667) antibody [EPR2319Y] |
Protocols for GCN2 (phospho T667) antibody [EPR2319Y] |
Price and availability of products related to GCN2 (phospho T667) antibody [EPR2319Y] |
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GCN2 (phospho T667) antibody [EPR2319Y] - related products:
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GCN2 (phospho T667) antibody [EPR2319Y] - other tools:
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Contact Abcam with a Technical Enquiry about GCN2 (phospho T667) antibody [EPR2319Y] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - GCN2 (phospho T667) antibody [EPR2319Y] (ab68427)](/index.html?datasheet=68427#GCN2_WB.gif)
