![Western blot - GCN2 (phospho T667) antibody [EPR2319Y] (ab68427) image](#GCN2_WB.gif)
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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Overview
Product name
Anti-GCN2 (phospho T667) antibody [EPR2319Y]
See all GCN2 products (5) ...
Description
Rabbit monoclonal [EPR2319Y] to GCN2 (phospho T667)
Specificity
Detects GCN2 only when phosphorylyated at threonine 667.
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding threonine 667 of human GCN2.
Positive control
HeLa cells
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 50mM Tris glycine, 0.15mM Sodium chloride, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR2319Y
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
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Western blot - GCN2 (phospho T667) antibody [EPR2319Y] (ab68427)
![Western blot - GCN2 (phospho T667) antibody [EPR2319Y] (ab68427) image](/ps/datasheet/Images/68/ab68427/GCN2_WB.gif)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab68427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/2000.Detects a band of approximately 220 kDa (predicted molecular weight: 220 kDa).
IP
IP: 1/30IP: 1/30
Application notes
Is unsuitable for ,Flow Cyt,ICC/IF or IHC-P.
Target
Function
Can phosphorylate the alpha subunit of EIF2 and may mediate translational control.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 protein kinase domains.
Contains 1 RWD domain.
Domain
Kinase domain 1 is a degenerate kinase domain.
RWD domain is reported to interact with GCN1L1.
Post-translational
modifications
Autophosphorylated on threonine residues.
Target information above from: UniProt accessionQ9P2K8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E2AK4_HUMAN antibody
- EIF2AK4 antibody
- Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody
see all
Database links
- Entrez Gene: 440275 Human
- Omim: 609280 Human
- SwissProt: Q9P2K8 Human
- Unigene: 656673 Human
Anti-GCN2 (phospho T667) antibody [EPR2319Y] images:
Western blot - GCN2 (phospho T667) antibody [EPR2319Y] (ab68427)
All lanes : Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427) at 1/2000 dilution
Lane 1 : HeLa cell lysate - untreated
Lane 2 : HeLa cell lysate - treated with LP
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution
Predicted band size : 220 kDa
Observed band size : 220 kDa
Additional bands at : 180 kDa. We are unsure as to the identity of these extra bands.
References for Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427)
ab68427 has not yet been referenced specifically in any publications.
Publishing research using ab68427? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"