Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
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All lanes : Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) at 1/2000 dilution
Lane 1 : Hela cell lysates un-treated
Lane 2 : Hela cell lysates treated with calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 187 kDa
Observed band size : 220 kDa (why is the actual band size different from the predicted?)
1
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