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Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)

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Overview

Product name

Anti-GCN2 (phospho T899) antibody [EPR2320Y]
See all GCN2 products (5) ...

Description

Rabbit monoclonal [EPR2320Y] to GCN2 (phospho T899)

Specificity

This antibody detects GCN2 phosphorylated at threonine 899.

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

A phospho specific peptide corresponding to residues surrounding threonine 899 of human GCN2

Positive control

Hela cell lysates

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EPR2320Y

Isotype

IgG

Research areas

Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation

  • Western blot - GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)Western blot - GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab75836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Is unsuitable for ,Flow Cyt,ICC/IF,IHC-P or IP.

Target

Function

Can phosphorylate the alpha subunit of EIF2 and may mediate translational control.

Tissue specificity

Widely expressed.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 protein kinase domains.
Contains 1 RWD domain.

Domain

Kinase domain 1 is a degenerate kinase domain.
RWD domain is reported to interact with GCN1L1.

Post-translational
modifications

Autophosphorylated on threonine residues.

Target information above from: UniProt accessionQ9P2K8 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • E2AK4_HUMAN antibody
  • EIF2AK4 antibody
  • Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody
  • Eukaryotic translation initiation factor 2-alpha kinase 4 antibody
  • GCN2 like protein antibody
  • GCN2-like protein antibody
  • KIAA1338 antibody
  • MGCN2 antibody
see all

Database links

Anti-GCN2 (phospho T899) antibody [EPR2320Y] images:

  Western blot - GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)

Western blot - GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)

All lanes : Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) at 1/2000 dilution

Lane 1 : Hela cell lysates un-treated
Lane 2 : Hela cell lysates treated with calyculin A

Lysates/proteins at 10 µg per lane.

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size : 187 kDa
Observed band size : 220 kDa (why is the actual band size different from the predicted?)

References for Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)

ab75836 has not yet been referenced specifically in any publications.

Publishing research using ab75836? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"