Anti-GCNT2 antibody (ab121888)
Key features and details
- Rabbit polyclonal to GCNT2
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GCNT2 antibody -
Description
Rabbit polyclonal to GCNT2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence, corresponding to amino acids 181-293 of Human GCNT2 Isoforms A and C (Q8N0V5, Q8NFS9).
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Positive control
- IHC-P: Human kidney tissue section. ICC/IF: Caco-2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
GCNT2 is the I-branching enzyme, a beta-1,6-N-acetylglucosaminyltransferase that converts linear into branched poly-N-acetyllactosaminoglycans and is responsible for the conversion of fetal i antigen to adult I antigen in erythrocytes during embryonic development. Mutations in this gene have been associated with adult i blood group phenotype. Alternatively spliced transcript variants encoding 3 different isoforms have been described. -
Cellular localization
Golgi apparatus membrane. -
Database links
- Entrez Gene: 2651 Human
- Omim: 600429 Human
- SwissProt: Q8N0V5 Human
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Alternative names
- bA360O19.2 antibody
- bA421M1.1 antibody
- Beta 1 6 N acetylglucosaminyltransferase 2 antibody
see all
Images
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Immunohistochemical analysis of human kidney tissue labelled for GCNT2 using ab121888. Positive staining observed in the golgi apparatus in cells in tubules .
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Immunohistochemical analysis of human duodenum tissue labelled for GCNT2 using ab121888. Positive staining observed in the golgi apparatus in glandular cells.
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Immunohistochemical analysis of human prostate tissue labelled for GCNT2 using ab121888. Positive staining observed in the golgi apparatus in glandular cells.
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Immunocytochemistry analysis of Caco-2 (human colorectal adenocarcinoma cell line) cells labelling GCNT2 with ab121888 at 2 µg/mL.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121888 has not yet been referenced specifically in any publications.