Anti-GCSF Receptor antibody (ab126167)
Key features and details
- Rabbit polyclonal to GCSF Receptor
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GCSF Receptor antibody
See all GCSF Receptor primary antibodies -
Description
Rabbit polyclonal to GCSF Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within internal sequence amino acids 164-392 of Human GCSF Receptor (UniProt Q99062).
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Positive control
- IHC-P: Human breast carcinoma tissue. IF: THP-1 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 92 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 92 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/500. |
Target
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Function
Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface. -
Tissue specificity
One or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U-937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U-937 cells. Isoform GCSFR-3 is highly expressed in placenta. -
Involvement in disease
Hereditary neutrophilia -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1441 Human
- Omim: 138971 Human
- SwissProt: Q99062 Human
- Unigene: 524517 Human
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Alternative names
- CD 114 antibody
- CD114 antibody
- CD114 antigen antibody
see all
Images
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Formalin-fixed, paraffin embedded human breast carcinoma tissue stained fro GCSF receptor usinf a126167 at 1/250 dilution in immunohistochemical analysis,
Antigen Retrieval: EDTA based, pH 8.0 buffer, 15min
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Immunofluorescence analysis of THP-1 (human monocytic leukemia cell line) cells labeling GCSF (green) using ab126167 at 1/500 dilution. Blue: Hoechst 33342 staining. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
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All lanes : Anti-GCSF Receptor antibody (ab126167) at 1/500 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 92 kDa7.5% SDS-PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab126167 has been referenced in 6 publications.
- Zhang JY et al. Vitamin D Promotes Trophoblast Cell Induced Separation of Vascular Smooth Muscle Cells in Vascular Remodeling via Induction of G-CSF. Front Cell Dev Biol 8:601043 (2020). PubMed: 33415106
- Magistroni V et al. De novo UBE2A mutations are recurrently acquired during chronic myeloid leukemia progression and interfere with myeloid differentiation pathways. Haematologica 104:1789-1797 (2019). PubMed: 30819912
- Dwivedi P et al. Phospho serine and threonine analysis of normal and mutated granulocyte colony stimulating factor receptors. Sci Data 6:21 (2019). PubMed: 30967555
- Zhang H et al. Unpaired Extracellular Cysteine Mutations of CSF3R Mediate Gain or Loss of Function. Cancer Res 77:4258-4267 (2017). PubMed: 28652245
- Maxson JE et al. The Colony-Stimulating Factor 3 Receptor T640N Mutation Is Oncogenic, Sensitive to JAK Inhibition, and Mimics T618I. Clin Cancer Res 22:757-64 (2016). WB ; Human . PubMed: 26475333
- Maxson JE et al. Ligand independence of the T618I mutation in the colony-stimulating factor 3 receptor (CSF3R) protein results from loss of O-linked glycosylation and increased receptor dimerization. J Biol Chem 289:5820-7 (2014). WB ; Human . PubMed: 24403076