GCSF Receptor protein (Active) (ab83994)
Constituents: 10% Trehalose, 1% Human serum albumin
Our Abpromise guarantee covers the use of ab83994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Molecular Mass: Under reducing conditions ab83994 migrates as a broad band between 100 and 130 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with unmodified ab83994 that has a predicted monomeric molecular mass of 93.9 kDa.
pI: Unmodified ab83994 has a predicted pI of 6.6.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- CD 114CD114CD114 antigen
- Colony stimulating factor 3 receptorColony stimulating factor 3 receptor (granulocyte)CSF 3RCSF3RCSF3R_HUMANCsfgrG CSF RG-CSF receptorG-CSF-RGCSFRGranulocyte colony stimulating factor receptorGranulocyte colony stimulating factor receptor precursorGranulocyte colony-stimulating factor receptorOTTHUMP00000009703OTTHUMP00000009704OTTHUMP00000009705
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
The box 1 motif is required for JAK interaction and/or activation.
GCSF Receptor protein (Active) images
1D SDS-PAGE of ab83994 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab83994
Lane 2: ab83994 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab83994 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
Approximately 5 µg of protein was loaded per lane; Gel was stained using Coomassie. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A tightening of the band after treatment with the glycosidase cocktail indicates that O-linked glycans may be present. Additional bands in lane 2 and lane 3 are glycosidase enzymes.
References for GCSF Receptor protein (Active) (ab83994)
ab83994 has not yet been referenced specifically in any publications.