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GCSF Receptor protein (Active) (ab83994)

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Overview

  • Product nameGCSF Receptor protein (Active)See all GCSF Receptor proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 25-621 of Human GCSF Receptor (Swiss-Prot Q99062) fused to the Fc region of human IgG1 (aa 90-330) expressed in modified human 293 cells.
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activityThe ED50 of ab83994 is typically 2-3 ng/ml as measured by its ability to neutralize GCSF mediated proliferation of the murine myeloblastic m-NFS-60 cell line.
  • FormLyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsShipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin
  • Concentration information loading...
  • Sequence notesTheoretical Sequence: ECGHISVSAPIVHLGDPITASCIIKQNCSHLDPEPQIL WRLGAELQPG GRQQRLSDGTQESIITLPHLNHTQAFLS CCLNWGNSLQILDQVELRAG YPPAIPHNLSCLMNLTTS SLICQWEPGPETHLPTSFTLKSFKSRGNCQ TQGDSILD CVPKDGQSHCCIPRKHLLLYQNMGIWVQAENALGTSMSPQ LCLDPMDVVKLEPPMLRTMDPSPEAAPPQAGCLQLCWE PWQPGLHINQ KCELRHKPQRGEASWALVGPLPLEALQY ELCGLLPATAYTLQIRCIRW PLPGHWSDWSPSLELRTT ERAPTVRLDTWWRQRQLDPRTVQLFWKPVP LEEDSGRI QGYVVSWRPSGQAGAILPLCNTTELSCTFHLPSEAQEVAL VAYNSAGTSRPTPVVFSESRGPALTRLHAMARDPHSLW VGWEPPNPWP QGYVIEWGLGPPSASNSNKTWRMEQNGR ATGFLLKENIRPFQLYEIIV TPLYQDTMGPSQHVYAYS QEMAPSHAPELHLKHIGKTWAQLEWVPEPP ELGKSPLT HYTIFWTNAQNQSFSAILNASSRGFVLHGLEPASLYHIHL MAASQAGATNSTVLTLMTLTPRSSNTKVDKKVEPKSCD KTHTCPPCPA PELLGGPSVFLFPPKPKDTLMISRTPEV TCVVVDVSHEDPEVKFNWYV DGVEVHNAKTKPREEQYN STYRVVSVLTVLHQDWLNGKEYKCKVSNKA LPAPIEKT ISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPS DIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVD KSRWQQGNVF SCSVMHEALHNHYTQKSLSLSPGK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab83994 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    Functional Studies
  • Application notesFuncS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Molecular Mass: Under reducing conditions ab83994 migrates as a broad band between 100 and 130 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with unmodified ab83994 that has a predicted monomeric molecular mass of 93.9 kDa.

    pI: Unmodified ab83994 has a predicted pI of 6.6.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        CD 114CD114CD114 antigen
        Colony stimulating factor 3 receptorColony stimulating factor 3 receptor (granulocyte)CSF 3RCSF3RCSF3R_HUMANCsfgrG CSF RG-CSF receptorG-CSF-RGCSFRGranulocyte colony stimulating factor receptorGranulocyte colony stimulating factor receptor precursorGranulocyte colony-stimulating factor receptorOTTHUMP00000009703OTTHUMP00000009704OTTHUMP00000009705
      see all
  • FunctionReceptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface.
  • Tissue specificityOne or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U937 cells. Isoform GSCFR-3 is highly expressed in placenta.
  • Involvement in diseaseDefects in CSF3R are the cause of hereditary neutrophilia (NEUTROPHILIA) [MIM:162830]. A form of lifelong, persistent neutrophilia, a condition characterized by an increase in the number of neutrophils in the blood.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 5 fibronectin type-III domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localizationSecreted and Cell membrane.

    • Target information above from: UniProt accession Q99062 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    GCSF Receptor protein (Active) images

    • SDS-PAGE - GCSF Receptor protein (Fc Chimera Active) (ab83994)
      SDS-PAGE - GCSF Receptor protein (Fc Chimera Active) (ab83994)
      1D SDS-PAGE of ab83994 before and after treatment with glycosidases to remove oligosaccharides.
      Lane 1: ab83994
      Lane 2: ab83994 treated with PNGase F to remove potential N-linked glycans
      Lane 3: ab83994 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
      Approximately 5 µg of protein was loaded per lane; Gel was stained using Coomassie.

      Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A tightening of the band after treatment with the glycosidase cocktail indicates that O-linked glycans may be present. Additional bands in lane 2 and lane 3 are glycosidase enzymes.

    References for GCSF Receptor protein (Active) (ab83994)

    ab83994 has not yet been referenced specifically in any publications.

    Publishing research using ab83994 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

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    Votes: Highest First

    I have recently purchased both the 25 ug and the 50 ug amounts of receptor. The instructions say to reconstitute with 0.5 ml of PBS. I need the relative amounts of HSA and receptor to say the same in my solution. Is the 25 ug sample just half of the 50 ug...

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    Each separate size is filled separately so that the target amount of protein of the primary product is maintained.
    When each is brought to 0.5ml, each will have only 1% HSA and 10% sugar as a stabilizer. The 25ug size will be 25ug/500ul and the ...

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    Do you have any data regarding the specific activity of your product in the NFS-60 proliferation assay?


    "We have our specification that was used to pass the current batch of material. The material would not have been released if it had not worked at the time of release and I've never had any complaints or reason to suspect otherwise.
    Our speci...

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    Do you have any data regarding whether HSA binds to your Fc GCSF-R? Also do you have data supporting the chimera's ability to dimerize in vitro?


    The source of the GCSF Receptor protein ab83994 informed me that :
    With regards to any of our proteins actually binding HSA, no we do not have any data, and I suppose one could get some minor binding just due to protein-protein interactions sinc...

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    Inquiry: Good evening, I have a question regarding the product ab83994 description. Under properties, it states "storage buffer constituents include 10% trehalose and 1% HSA" Does that mean that the lyophilised product also contains these additi...

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    Thank you for your inquiry.

    I can confirm that 10% Trehalose and 1% Human serum albumin are contained in the lyophilized product.

    Please follow the instructions on the datasheet for reconstitution.

    I hope this information is ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"