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Overview

  • Product nameAnti-GDI2 antibodySee all GDI2 primary antibodies ...
  • Description
    Rabbit polyclonal to GDI2
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow, Dog, Pig, Caenorhabditis elegans
  • Immunogen

    A region within synthetic peptide: EPEKEIRPAL ELLEPIEQKF VSISDLLVPK DLGTESQIFI SRTYDATTHF, corresponding to amino acids 361-410 of Human GDI2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlHepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab49193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 0.5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Alternative names
      anti GDI2 antibodyFLJ16452 antibodyFLJ37352 antibody
      GDI 2 antibodyGDI-2 antibodyGDI2 antibodyGDI2 antibody antibodyGDIB_HUMAN antibodyGDP dissociation inhibitor 2 antibodyGuanosine diphosphate dissociation inhibitor 2 antibodyRab GDI beta antibodyRab GDP dissociation inhibitor beta antibodyRABGDIB antibody
    see all

Anti-GDI2 antibody images

  • Western blot - GDI2 antibody (ab49193)
    Western blot - GDI2 antibody (ab49193)
    All lanes : Anti-GDI2 antibody (ab49193) at 0.5 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution
    developed using the ECL technique

    Predicted band size : 51 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

References for Anti-GDI2 antibody (ab49193)

ab49193 has not yet been referenced specifically in any publications.

Publishing research using ab49193 ? Please let us know so that we can cite the reference in this datasheet

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Western blot abreview for Anti-GDI2 antibody

 Good
Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (astrocytroma)
Loading amount 25 µg
Specification astrocytroma
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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Submitted Apr 10 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"