GDNF Receptor alpha 1 antibody (ab8026)

Product Name 

GDNF Receptor alpha 1 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to GDNF Receptor alpha 1

Immunogen 

Synthetic peptide, corresponding to amino acids 369-382 of Human GFR alpha 1.

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

IHC (PFA fixed), IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval (citrate buffer 10 mM, pH 6.0) before commencing with IHC staining protocol.
Immunohistochemistry (PFA fixed): 1/50.
WB: 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human brain lysate.

Cellular localization 

Cell Membrane

Research areas 

Neuroscience >> Neurology process >> Growth and Development >> Neurotrophins

Relevance 

Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of kidneys and the enteric nervous system. Physiological responses to GDNF require the presence of a novel glycosylphosphadidylinositol linked protein GDNFRa, which is a cell surface receptor for GDNF. The cDNAs encoding GDNFRa from human, rat, chicken and mouse have been cloned recently. GDNFRa was also termed Ret ligand 1 (RETL1) or TGF b related neurotrophic factor receptor 1 (TrnR1) and nominated as GFRa 1. GFRa 1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus, GDNF, GFRa and the Ret PTK form a complex to transduce GDNF signal and to mediate GDNF function.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

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References for GDNF Receptor alpha 1 antibody (ab8026)

  This product has been used in: (two most recent references)

He Z et al. Isolation, characterization, and culture of human spermatogonia. Biol Reprod 82:363-72 (2010). IHC-P; Human. PubMed: 19846602

Serra MP et al. Ret, GFRalpha-1, GFRalpha-2 and GFRalpha-3 receptors in the human hippocampus and fascia dentata. Int J Dev Neurosci 23:425-38 (2005). PubMed: 16002253
If you publish research using ab8026 please let us know so that we can cite the reference on this datasheet.

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