Anti-GEF H1 antibody (ab155785)
Key features and details
- Rabbit polyclonal to GEF H1
- Suitable for: IP, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GEF H1 antibody
See all GEF H1 primary antibodies -
Description
Rabbit polyclonal to GEF H1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 656-1000 of Human GEF H1.
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Positive control
- 293T, A431, HeLa, hMSC-3A6, RAW264.7 and C2C12 whole cell lysates; GEF H1 protein; HeLa cells; Human gastric carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP | (1) |
Use at an assay dependent concentration.
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WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 111 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF | (1) |
1/100 - 1/1000.
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Notes |
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IP
Use at an assay dependent concentration. |
WB
1/500 - 1/3000. Predicted molecular weight: 111 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in PubMed:9857026. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA. -
Sequence similarities
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger. -
Domain
The DH (DBL-homology) domain interacts with and promotes loading of GTP on RhoA.
The PH (pleckstrin-homology) domain is involved in microtubule binding and targeting to tight junctions. -
Post-translational
modificationsPhosphorylation of Ser-886 by PAK1 induces binding to protein 14-3-3 zeta, promoting its relocation to microtubules and the inhibition of its activity. Phosphorylated by STK6 and CDK1 during mitosis, which negatively regulates its activity. Phosphorylation by MAPK1 or MAPK3 increases nucleotide exchange activity. Phosphorylation by PAK4 releases GEF-H1 from the microtubules. -
Cellular localization
Cytoplasm. Cell junction > tight junction. Golgi apparatus. Cytoplasm > cytoskeleton > spindle. Cell projection > ruffle membrane. Localizes to the tips of cortical microtubules of the mitotic spindle during cell division, and is further released upon microtubule depolymerization. Recruited into membrane ruffles induced by S.flexneri at tight junctions of polarized epithelial cells. - Information by UniProt
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Database links
- Entrez Gene: 9181 Human
- Entrez Gene: 16800 Mouse
- Omim: 607560 Human
- SwissProt: Q92974 Human
- SwissProt: Q60875 Mouse
- Unigene: 516790 Human
- Unigene: 239329 Mouse
- Unigene: 482396 Mouse
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Alternative names
- AA408978 antibody
- ARHG2 antibody
- ARHG2_HUMAN antibody
see all
Images
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All lanes : Anti-GEF H1 antibody (ab155785) at 1/2000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 111 kDa
5% SDS PAGE -
Immunocytochemical immunofluorescence analysis of formaldehyde-fixed human HepG2 cells, labelling GEF H1 with ab155785 with a dilution of 1/50 incubated for 3 hours at 22°C in 1% donkey serum in 0.1% PBST diluent. Permeablization with 0.2% Triton X-100. Blocking was with 1% donkey serum in 0.1% PBST incubated for 1 hour at 22°C. Secondary was a donkey anti-rabbit polyclonal Alexa Fluor® 594 at 1/200. Counterstain is DAPI (blue) against nuclear DNA.
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Immunohistochemical analysis of paraffin-embedded Human Gastric carcinoma tissue, labeling GEF H1 with ab155785 at 1/500 dilution.
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Immunoprecipitation of GEF H1 from 293T whole cell extracts using 5 μg of ab155785. Western blot analysis was performed using ab155785.
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Immunohistochemical analysis of paraffin-embedded Mouse testis tissue, labeling GEF H1 with ab155785 at 1/500 dilution.
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All lanes : Anti-GEF H1 antibody (ab155785) at 1/1000 dilution
Lane 1 : hMSC-3A6 whole cell lysate, mock transfected
Lane 2 : hMSC-3A6 whole cell lysate, transfected with GEF H1 siRNA
Lysates/proteins at 10 µg per lane.
Predicted band size: 111 kDa
8% SDS PAGE -
All lanes : Anti-GEF H1 antibody (ab155785) at 1/1000 dilution
Lane 1 : RAW264.7 whole cell lysate
Lane 2 : C2C12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 111 kDa
5 % SDS-PAGE -
Immunofluorescent analysis of methanol-fixed (-20°C 100% MeOH for 5 min) HeLa cells, labeling GEF H1 with ab155785 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab155785 has been referenced in 23 publications.
- Seetharaman S et al. Microtubules tune mechanosensitive cell responses. Nat Mater 21:366-377 (2022). PubMed: 34663953
- Leguay K et al. Interphase microtubule disassembly is a signaling cue that drives cell rounding at mitotic entry. J Cell Biol 221:N/A (2022). PubMed: 35482006
- Zuidema A et al. Molecular determinants of αVβ5 localization in flat clathrin lattices - role of αVβ5 in cell adhesion and proliferation. J Cell Sci 135:N/A (2022). PubMed: 35532004
- Yuan L et al. Lateral confined growth of cells activates Lef1 dependent pathways to regulate cell-state transitions. Sci Rep 12:17318 (2022). PubMed: 36243826
- Chen X et al. Androgen deprivation restores ARHGEF2 to promote neuroendocrine differentiation of prostate cancer. Cell Death Dis 13:927 (2022). PubMed: 36335093