Anti-GFAP antibody - Astrocyte Marker (ab4674)

Antibody does not stain GFAP in Fresh Frozen Brain sections.

ANSWER:

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on the phone, I amattachning our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

Oh really that’s great! To confirm, that the primary antibody AB4674 also qualifies for a testing discount?



Sorry I forgot to include the customer’s details.




Please let me know if you require any further information

ANSWER:

DISCOUNT CODE for ab4674 Anti-GFAP antibody - Astrocyte Marker: XXXXX
DISCOUNT CODE for ab46969 Goat anti-Chicken IgY H&L (FITC) secondary antibody: YYYYYYY


Expiration date: ZZZZZZZ

I am very pleased to hear your customer would like to test bothab4674 andab46969 in Flow Cytometry. The code will give them 1 free primary antibody and 1 secondary antibody before the expiration date. To redeem this offer, please submit an Abreview for Flow Cytometry and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

I would like to know, why the GFAP (ab4674) has no BSA as a carrier Protein. Doesn’t the Antibody bind unspecifically then? If I store the antibody at -20° C how long can I store it? regards

ANSWER:

Thank you for your enquiry. I can confirm this antibody is BSA free. BSA is added to the storage buffer when the antibody is a lower concentration. The additional protein provided by the addition of BSA stabilizes the antibody. This is unnecessary in case ofab4674 since this product shows a very high antibody concentration of 33mg/ml. BSA in the storage buffer is not connected to high background and unspecific binding during the experiment. Even when antibodies contain BSA we recommend to always include a blocking steop during the experiment. We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at leastsix monthfrom the date of purchase when stored correctly. For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (http://www.abcam.com/index.html?pageconfig=popular_protocols). I hope this information helps. Please do not hesitate to contact us if you need anything further.

Abreview testing codes have expires prematurely.

ANSWER:

Thank you for your call today, and I apologize for the trouble with these codes! I have created new codes which will expire on March 1, 2012. I will go through your Abreviews and put these new codes in our internal notes so that the discounts can be applied to your orders tomorrow. Please let me know if you have any questions, or if there is anything else that we can do for you.

ab4674 produces non-specific staining in my perfused-fixed mouse sciatic nerve sections

ANSWER:

Thank you for your phone call and for providing your protocol information. I am sorry that this antibody is producing non-specific staining in your experiments. I would be happy to send you an alternative GFAP antibody as a free of charge replacement. Please let me know which product you would like to receive and I will process your request.