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Products:Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments
Overview
Product name
Anti-GFAP antibody - Astrocyte Marker
See all GFAP products (35) ...
Description
Chicken polyclonal to GFAP - Astrocyte Marker
Tested applications
IHC-Fr, IHC-FoFr, IHC-P, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Cow, Cat, Human, Rhesus monkey, Apteronotus leptorhynchus
Predicted to work with
all Mammals
Immunogen
Full length native protein (purified from a Triton X-100 extract of myelin associated material, further purified by centrifugation and ion exchange chromatography in 6m urea on DEAE cellulose) (Cow).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgY
Research areas
Stem Cells >> Neural Stem Cells >> Glial Restricted Lineage >> Astrocyte
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class II >> GFAP
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/1000 - 1/5000.(Try this antibody at about between about 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme linked methods.)
IHC-FoFr: 1/1000 - 1/5000.(PubMed: 20098733Try this antibody at about between about 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme linked methods.)
IHC-P: 1/1000 - 1/5000.(Try this antibody at about between about 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme linked methods.)
WB: 1/50000 - 1/100000.Predicted molecular weight: 50 kDa.(Expect to see a band at 55kDa and another at about 48kDa, apparently a breakdown product of the 55kDa band.)
ICC/IF: 1/1000 - 1/5000.(Try this antibody at about between about 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme linked methods.)
Target
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Tissue specificity
Expressed in cells lacking fibronectin.
Involvement in disease
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
Sequence similarities
Belongs to the intermediate filament family.
Post-translational
modifications
Phosphorylated by PKN1.
Cellular localization
Cytoplasm. Associated with intermediate filaments.
Target information above from: UniProt accessionP14136
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Astrocyte antibody
- FLJ42474 antibody
- FLJ45472 antibody
see all
Database links
- Entrez Gene: 419969 Chicken
- Entrez Gene: 281189 Cow
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Entrez Gene: 24387 Rat
- Omim: 137780 Human
- SwissProt: Q28115 Cow
- SwissProt: P14136 Human
see all
Anti-GFAP antibody - Astrocyte Marker images:
Immunocytochemistry/ Immunofluorescence - GFAP antibody - Astrocyte Marker (ab4674)
Rat astrocytes stained with ab4674 (red). The anti-GFAP antibody was used at a dilution of 1:50 from affinity purified material using our standard fixation and staining procedure (in protocol section). Hoechst dye reveals nuclear DNA in blue.
Immunohistochemistry (PFA perfusion fixed frozen sections) - anti-GFAP antibody - Astrocyte Marker (ab4674)
ab4674 staining GFAP in mouse hippocampus tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with 4% PFA and blocking with 10% serum for 30 minutes at 250C was performed. The sample was incubated with primary antibody (1/500) for 16 hours at 250C in 10% NGS in PBS + 0.1% TX100. An Alexa Fluor®488-conjugated Goat polyclonal to chicken IgG was used as secondary antibody at 1/400 dilution. Staining was intensified with 2-3 minutes of retrieval with trypsin at room temperature.
This image is a courtesy of Ben Deverman
References for Anti-GFAP antibody - Astrocyte Marker (ab4674)
This product has been referenced in:
- Lioy DTet al. A role for glia in the progression of Rett's syndrome. Nature 475:497-500 (2011). IHC-FoFr; Mouse.Read more (PubMed: 21716289) »
- Afshari FTet al. Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling. J Neurosci 30:4246-55 (2010). ICC/IF; Rat.Read more (PubMed: 20335460) »
See all 15 publications for this product
Publishing research using ab4674? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"