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Immunocytochemistry/ Immunofluorescence - GFAP antibody - Astrocyte Marker (ab4674)
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Immunohistochemistry (PFA perfusion fixed frozen sections) - GFAP antibody - Astrocyte Marker (ab4674)
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Product Name
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GFAP antibody - Astrocyte Marker
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See all GFAP antibodies (29)...
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Product type
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Primary antibodies
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Description
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Chicken polyclonal to GFAP - Astrocyte Marker
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Immunogen
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Full length native protein (purified from a Triton X-100 extract of myelin associated material, further purified by centrifugation and ion exchange chromatography in 6m urea on DEAE cellulose) (Cow).
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Reacts with
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Hu, Ms, Rat, Cat, Chk, Cow
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Predicted to react
(species key)
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Mamm
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/2000.
IHC-Fr: 1/50.
IHC-FoFr: Use at an assay dependent dilution (PMID 20098733).
WB: 1/10000. Predicted molecular weight: 50 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Stem Cells >> Neural Stem Cells >> Glial Restricted Lineage >> Astrocyte
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class II >> GFAP
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments
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Relevance
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Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Chicken
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Clonality
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Polyclonal
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Isotype
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IgY
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: PBS
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Form
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Liquid
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Concentration
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33.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GFAP antibody - Astrocyte Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GFAP antibody - Astrocyte Marker images, references, products related to ab4674 and other tools.
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GFAP antibody - Astrocyte Marker images:
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 Immunocytochemistry/ Immunofluorescence - GFAP antibody - Astrocyte Marker (ab4674)
Rat astrocytes stained with ab4674 (red). The GFAP antibody was used at a dilution of 1:50 from affinity purified material using our standard fixation and staining procedure (in protocol section). Hoechst dye reveals nuclear DNA in blue.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - GFAP antibody - Astrocyte Marker (ab4674)
ab4674 staining GFAP in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with 4% PFA and blocked with 10% serum for 30 minutes at 250C. The sample was incubated with primary antibody (1/500) for 16 hours at 250C in 10% NGS in PBS + 0.1% TX100. An Alexa Fluor®488-conjugated Goat polyclonal to chicken IgG (1/400) was used as secondary antibody. This image is a courtesy of Ben Deverman
See Abreview
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References for GFAP antibody - Astrocyte Marker (ab4674)
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This product has been used in: (two most recent references)
Pan-Montojo F et al. Progression of Parkinson's disease pathology is reproduced by intragastric administration of rotenone in mice. PLoS One 5:e8762 (2010). IHC-FoFr; Mouse. PubMed: 20098733
Gadea A et al. Endothelin-1 regulates astrocyte proliferation and reactive gliosis via a JNK/c-Jun signaling pathway. J Neurosci 28:2394-408 (2008). PubMed: 18322086
See all 10 publication references for this product.
If you publish research using ab4674 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to GFAP
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GFAP antibody - Astrocyte Marker - more information
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GFAP antibody - Astrocyte Marker - related products:
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GFAP antibody - Astrocyte Marker - other tools:
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Contact Abcam with a Technical Enquiry about GFAP antibody - Astrocyte Marker |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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