Products:Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments
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ab7779 has been referenced in 8 publications.
Publishing research using ab7779? Please let us know so that we can cite the reference in this datasheet
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ab7779 at a dilution of 1/1000, staining GFAP in astrocytes (Alexa 488 secondary at 1/2000) on 30
NB: No labeling observed following omission of primary antibody.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
ab7779 staining human brain tissue by ICC/IF. The section was formaldehyde fixed and blocked with 3% serum (for 1 hour at 24°C) prior to incubation with ab7779 (1/1000) for 12 hours at 24°C. An Alexa Fluor® 546 conjugated goat anti-rabbit was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Anti-GFAP antibody - Astrocyte Marker (ab7779) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 50 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
4
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