![Western blot - GFAP antibody [EP672Y] (ab33922) image](#33922.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - GFAP antibody [EP672Y] (ab33922) image](#33922a.jpg)
![Immunohistochemistry (Frozen sections) - GFAP antibody [EP672Y] (ab33922) image](#GFAP-Primary-antibodies-ab33922-5.jpg)
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Product name
Anti-GFAP antibody [EP672Y]
See all GFAP products (36) ...
Description
Rabbit monoclonal [EP672Y] to GFAP
Tested applications
IHC-Fr, WB, IHC-P, IP, ICCmore details
Cross reactivity
Reacts with
Rat, Human
Does not react with
Mouse
Immunogen
A synthetic peptide corresponding to 405 - 432aa in the C terminus of GFAP (Human)
Positive control
Rat brain lysate, Human brain
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
EP672Y
Isotype
IgG
Research areas
Stem Cells >> Neural Stem Cells >> Glial Restricted Lineage >> Astrocyte
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class II >> GFAP
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments
Applications
Show applications key
Our Abpromise guarantee covers the use of ab33922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/250 - 1/1000.
WB: 1/2000. Predicted molecular weight: 50 kDa.
IHC-P: 1/250 - 1/500.
IP
IP: 1/50.IP: 1/50.
ICC
ICC: 1/250 - 1/500.ICC: 1/250 - 1/500.
Application notes
Is unsuitable for or Flow Cyt.
Target
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Tissue specificity
Expressed in cells lacking fibronectin.
Involvement in disease
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
Sequence similarities
Belongs to the intermediate filament family.
Post-translational
modifications
Phosphorylated by PKN1.
Cellular localization
Cytoplasm. Associated with intermediate filaments.
Target information above from: UniProt accessionP14136
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Astrocyte antibody
- FLJ42474 antibody
- FLJ45472 antibody
see all
Database links
- Entrez Gene: 2670 Human
- Entrez Gene: 24387 Rat
- Omim: 137780 Human
- SwissProt: P14136 Human
- SwissProt: P47819 Rat
- Unigene: 514227 Human
- Unigene: 91512 Rat
Anti-GFAP antibody [EP672Y] images:
Western blot - GFAP antibody [EP672Y] (ab33922)
![Western blot - GFAP antibody [EP672Y] (ab33922)](/ps/datasheet/Images/33/ab33922/33922.jpg)
Anti-GFAP antibody [EP672Y] (ab33922) at 1/2000 dilution + Rat brain lysate
Predicted band size : 50 kDa
Observed band size : 50 kDa
Immunohistochemistry (Paraffin-embedded sections) - GFAP antibody [EP672Y] (ab33922)
![Immunohistochemistry (Paraffin-embedded sections) - GFAP antibody [EP672Y] (ab33922)](/ps/datasheet/Images/33/ab33922/33922a.jpg)
Immjunohistochemical analysis of paraffin-embedded human brain using ab33922 at 1/250.
Immunohistochemistry (Frozen sections) - GFAP antibody [EP672Y] (ab33922)
![Immunohistochemistry (Frozen sections) - GFAP antibody [EP672Y] (ab33922)](/ps/datasheet/images/33/ab33922/GFAP-Primary-antibodies-ab33922-5.jpg)
ab33922 staining GFAP in a xenograft section, human neural stem cells in fetal mouse, by Immunohistochemistry (Frozen sections).Tissue was fixed in paraformaldehyde and permeabilized with 0.2% Triton X. Samples were then blocked using 3% normal donkey serum for 1.5 hours at 20°C, then incubated with ab33922 at a 1/1000 dilution for 1 hour at 20°C. The secondary used was a donkey anti-rabbit polyclonal conjugated to Alexa Fluor 555, used at a 1/500 dilution.ab33922 only recognizes human astrocytes and none of the murine tissue was stained. The staining of the human GFAP fibers was always very strong, clear and reliable. Very good antibody for xenograft experiments in neuroscience!
Image courtesy of Silvan Francke by Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-GFAP antibody [EP672Y](ab33922)
](/ps/datasheet/images/33/ab33922/GFAP-Primary-antibodies-ab33922-6.jpg)
ab33922 showing positive staining in Normal brain tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-GFAP antibody [EP672Y](ab33922)
](/ps/datasheet/images/33/ab33922/GFAP-Primary-antibodies-ab33922-7.jpg)
ab33922 showing positive staining in Astrocytoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-GFAP antibody [EP672Y](ab33922)
](/ps/datasheet/images/33/ab33922/GFAP-Primary-antibodies-ab33922-8.jpg)
ab33922 showing negative staining in Meningioma tissue.
References for Anti-GFAP antibody [EP672Y] (ab33922)
ab33922 has not yet been referenced specifically in any publications.
Publishing research using ab33922? Please let us know so that we can cite the reference in this datasheet
![GFAP antibody [EP672Y] for Immunohistochemistry (Frozen sections) in Human (33922)](/ps/datasheet/images/33/ab33922/GFAP-Primary-antibodies-ab33922-4.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"